Treatment of spinach chloroplasts with 25 to 100 pM potassium permanganate in the presence of light, magnesium, and ADP gives rise to 35 to 60% inhibition of the subsequent phosphorylation and ATPase activities. For half-maximal inhibition, 5 to 10 PM ADP is necessary and ADP is not replaceable by other nucleotides. The light requirement can be met by preillumination as well as by an acid bath ...

During the early phases of tissue sectioning for electron microscopy, virtually all of the standard cytological fixatives were tried. Of these, osmium tetroxide appeared to give the best preservation, and this was further improved by buffering the fixative solution at a slightly alkaline pH (Palade (1)). A variant developed by Dalton (2) contains Os04 in a chromate-dichromate system. Formalin h...

III.?The Epidemic of 1895. In this outbreak I introduced Hankin's permanganate of potassium treatment solely. I have made an endeavour, in the map accompanying this report of the last cholera epidemic here, to show as far as possible the number of wells solely or chiefly used by those persons who were attacked, and which were in consequence disinfected (marked " P," the figures indicating cases...

animals with a view to determining the efficacy of this agent, administered in this fashion, and fixing a dose." Later in the same paper the author recommends " the intravenous injection of 350 c.c. of a 5 per cent, solution of permanganate of potash " in cases of snake-poisoning in human beings. It is against this procedure that I wish to warn the medical profession, and the urgency of the mat...

THE CLASSICAL PROCEDURE for the determination of calcium in biologic fluids involves the precipitation of calcium as the oxalate and titration of the oxalate with potassium permanganate. The permanganate titration has certain difficulties. The oxalate solution has to be heated during the titration (1) and the end point is unreliable in dilute solutions (2). The use of cerate oxidimetry avoids t...

Chemiluminescence from the vanadium and potassium permanganate reaction was studied under acidic conditions to develop a sensitive method for vanadium determination using the formaldehyde enhancement effect. The method was successfully applied to the determination of vanadium in seawater. Experimental parameters were optimized, including acid concentration, potassium permanganate and formaldehy...

The density of sympathetic nerve terminals in human superficial temporal arteries from 5 cases at intra- and extracranial bypass surgery was examined with two histochemical methods, one with potassium permanganate fixation and the other with the new monoamine oxidase staining technique. By potassium permanganate fixation, small cored vesicles containing fibers of noradrenergic nerve terminals m...

1. Nitric oxide that was produced by reducing nitrite with an excess of acidified potassium iodide under nitrogen in Warburg respirometer flasks was rapidly absorbed by a solution of permanganate in sodium hydroxide held in the side arm. A small amount of nitrous oxide (or nitrogen) that was also produced was not absorbed. 2. By using a quantitative method for the recovery of nitrite from sampl...

An indirect, sensitive and accurate method for the determination of trace amounts of hydrazine is described. In this proposed the spectrophotometric method is based on its reduction properties of hydrazine with a known concentration of potassium permanganate to reduce the colour. The absorbance of unreduced permanganate is measured the colour difference at different wavelengths 546 and 526 nm w...