In assisted reproductive technology (ART), the pregnancy and birth rates following in vitro fertilization (IVF) attempts are still low. Recently, apoptotic markers have been suggested as new criteria for oocyte and embryo quality selection. Many studies have provided evidence that poor oocyte and embryo quality can be associated with apoptosis. The aim of this review is to summarize our current...

Abstract Study question Is a change of embryo quality through vitrification and thawing associated with reduced LBR when single euploid frozen transfer is performed? Summary answer Live birth rates are not influenced by in cryopreservation transfer. What known already Embryo aneuploidy the most significant factor accounts for about 50% implantation failure miscarriage human reproduction. Preimp...

Background According to the global increasing trend of infertility, using assisted reproductive technologies to solve this issue is recommended. It seems that vitamin E is an antioxidant with protective role in the body. Therefore, we aimed to investigate the relation between levels of vitamin E in Follicular fluid with the morphology of oocytes and the quality of embryos in patients with IVF. ...

Background: Development of cloned bovine embryos. Materials and Methods: Oocytes collection,oocyte denudation, oocyte enucleation, nuclear transfer, electrofusion, reconstructed embryo activation, culture of reconstructed and IVF embryo,and treatment with SAH post fusion interval Treatment of reconstructed embryos with TSA for 12 hours after activation, preparation of somatic donor cells, donor...

Modifying cryopreservation protocols may be seen as a way to simplify cryobanking procedure and increase satisfying outcomes. The aim of the study was to evaluate the influence of slow cooling protocol and vitrification on IVF outcomes using embryos preserved in the 5th or 6th day after oocyte retrieval. The study compared 2 groups of human embryos underwent slow cooling protocol (n=189) and vi...

Background/aim: The aim of this study was to evaluate the efficiency in vitro embryo splitting (IES) procedures. We also assessed quality blastocysts developed from embryos obtained different sources. Materials and methods: Good at 6-8-cell stages were categorized according their fertilization sources: 1) frozenwarmed donated embryos, 2) chromosomally abnormal 3) parthenogenetic 4) derived matu...

background: this study was designed to develop a new co-culture system, assess the effect of luteinizing hormone (lh) using sequential media to promote development and increase the quality of 2-cell murine embryos through the 8-16 cell stage to morula and blastocyst stages. materials and methods: monolayers for co-culture were prepared from ovine oviduct epithelial cells (ooec) in dmem/f12 medi...

Background: Currently, a limited number of studies have been done on the vitrification of small ruminant oocytes, especially in sheep, where poor developmental rates are obtained following immature and mature oocyte vitrification. Poor ovine oocyte cryopreservation could attribute to the damage to enzymes which has role in metabolic pathways. Therefore, production of cytokines and growth factor...

BACKGROUND Quality control programs are necessary to maintain good clinical practice. Embryo grading has been described as one of the external quality assurance schemes. Although the evaluation of embryos is based on the assessment of morphological characteristics, considerable intra- and inter-observer variability has been described. In this multicentre study, the variability in the embryo eva...