Waveguide numerical aperture restrictions and light-blocking elements are used to create a microfluidic cytometer with both illumination and two-parameter light scatter collection systems integrated on-chip. Good forward scatter coefficients of variation (9.7-18.3%) are achieved for polystyrene beads under a reasonably high flow rate (28 cm/s) using a greatly simplified optical system.

The authors present dielectrophoresis DEP -based tweezers that can be used to characterize the interactions between a particle and the surface it is attached to, within a microfluidic device. As a proof of concept, 5.4 m polystyrene beads functionalized by carboxyl group were attached on a bare and poly-L-lysine functionalized oxide surface. Negative dielectrophoresis force was generated using ...

A latex agglutination test (LAT) based on polystyrene beads sensitized with inactivated avian influenza virus H5N1 particles was developed. Compared with the hemagglutination inhibition test, the sensitivity and specificity of the LAT were 88.8 and 97.6%, respectively, in detecting 830 serum samples from vaccinated chickens. The test has application potential in field practice.

By spectral phase shaping of both the pump and probe pulses in coherent anti-Stokes Raman scattering (CARS) spectroscopy we demonstrate the extraction of the frequencies of vibrational lines using an unamplified oscillator. Furthermore we demonstrate chemically selective broadband CARS microscopy on a mixed sample of 4 μm diameter polystyrene (PS) and poly(methyl methacrylate) (PMMA) beads. The...

A library of heptapeptides displayed on the surface of filamentous phage M13 was evaluated as a potential source of affinity ligands for the purification of Rhizomucor miehei lipase. Two independent selection (biopanning) protocols were employed: the enzyme was either physically adsorbed on polystyrene or chemically immobilized on small magnetic beads. From screening with the polystyrene-adsorb...

The circulating leucocytes of insects, the haemocytes, adhere to and encapsulate foreign material that enters the insect's body cavity. The thickness of the capsule depends not only on the insect species but also on the nature of the object concerned, a fact that is of great importance to invading parasites and pathogens. In this paper, some of the factors that may stimulate haemocyte adhesion ...

We report an approach to fabricate dual-faced polystyrene beads (DFPSBs) with trifunctions of tumor cells recognition, drug delivery, and real-time Raman sensing. One-step oxygen-plasma treatment process was used to etch commercially available fluorescent polystyrene beads into a corrugated upper hemisphere and simultaneously change the entire surface with carboxylic groups. After depositing go...

We introduce a method for detecting and tracking small particles in a solution near a surface. The method is based on blocking the backreflected illumination beam in an objective-type total internal reflection microscope, leaving unhindered the light scattered by the particles and resulting in dark-field illumination. Using this method, we tracked the motion of 60-nm polystyrene beads with a si...