Genomic DNA extraction with a high quantity and quality is a basic requirement in molecular biology. The DNA obtained was free of any contamination proteins, polysaccharide, polyphenols and colored pigments. These compounds would interfere with the genomic isolation procedures and downstream reactions such as restriction enzyme analysis and gene amplification. The isolated genomic DNA was fo...

Immune status of apparently healthy Nepalese against hemolytic streptococci was studied by measuring antibodies to various streptococcal antigens such as streptolysin-O (SO), streptokinase (SK), deoxyribonuclease-B (DN-B) and streptococcal polysaccharide (SP). The normal range of anti-streptolysin-O (ASO), anti-streptokinase (ASK), anti-deoxyribonuclease-B (ADN-B) and anti-Streptococcus polysac...

1. A procedure for the isolation and purification of the heparin-protein complex from ox liver capsule, based on the solubility properties of mucopolysaccharide-cetylpyridinium complexes, is described. 2. The yield of the heparin-protein complex with this method averages 35mg./100g. of dry ox liver capsule. 3. The results of analyses on the polysaccharide show good agreement with values previou...

Acinetobacter calcoaceticus BD4 and BD413 produce extracellular emulsifying agents when grown on 2% ethanol medium. For emulsifying activity, both polysaccharide and protein fractions were required, as demonstrated by selective digestion of the polysaccharide with a specific bacteriophage-borne polysaccharide depolymerase, deproteinization of the extracellular emulsifying complex with hot pheno...

Periodate oxidation is one of the most important reaction in structural study of non-ionic polysaccharide. Periodate oxidation was done using sodium metaperiodate as oxidant proposed by Fluery & Lange’s method. It consumed 1.16 moles of periodate and liberated 0.23 moles of formic acid per mole of anhydrohexose unit after 50 hrs. The isolated water soluble seeds extract yielded sugars as D-gala...

A novel method to control polythiophene redox potentials based on supramolecular complexation with the native polysaccharide, schizophyllan (SPG) is reported, which can importantly improve air stability for easy handling and processing.