Sequence databases could be efficiently exploited for development of DNA markers if it were known which gene regions reveal the most polymorphism when amplified by PCR. We developed PCR primer pairs that target specific regions of previously sequenced genes from Avena and Zea species. Primers were targeted to amplify 40 introns, 24 exons, and 23 promoter regions within 54 maize genes. We survey...

PREMISE OF THE STUDY Polymorphic microsatellite markers of Zanthoxylum schinifolium (Rutaceae), a promising medicinal plant with effective antibacterial, anticancer, and anti-inflammatory compounds, were developed and evaluated for further genetic studies based on genetic variation among individuals or populations. METHODS AND RESULTS Following the selective hybridization method, microsatelli...

PREMISE OF THE STUDY Nonplastid microsatellite primers were developed for the first time in the Euro-Siberian complex of Anthoxanthum (Poaceae), a genus of temperate grasses in which reticulate evolution is common. METHODS AND RESULTS A microsatellite-enriched genomic DNA library allowed the detection of 500 fragments containing a microsatellite motif. Fifteen primer pairs were selected for a...

the genetic diversity among 56 almond (prunus dulcis) genotypes was analysed using 35 microsatellite markers and 14 morphological traits. analysis of morphological traits revealed a wide range of variation among the studied genotypes. out of 35 simple sequence repeats (ssrs) markers, 25 were polymorphic, producing 215 alleles that varied from 2 to 16 with an average of 8.76 alleles per locus. r...

A consensus map for sugi (Cryptomeria japonica) was constructed by integrating linkage data from two unrelated third-generation pedigrees, one derived from a full-sib cross and the other by self-pollination of F1 individuals. The progeny segregation data of the first pedigree were derived from cleaved amplified polymorphic sequences, microsatellites, restriction fragment length polymorphisms, a...

We have completed a second-generation linkage map that incorporates sequence-based positional information. This new map, the Rutgers Map v.2, includes 28,121 polymorphic markers with physical positions corroborated by recombination-based data. Sex-averaged and sex-specific linkage map distances, along with confidence intervals, have been estimated for all map intervals. In addition, a regressio...

Extensive molecular studies have characterized 15 dimorphic and 2 multiallelic genetic markers within the human alpha-globin gene cluster. Analysis of these markers in 9 populations has shown that the alpha-globin locus is remarkably polymorphic and is therefore an ideal marker on chromosome 16 for the construction of a human genetic linkage map. The combined analysis of 9 polymorphic markers h...

in the present study, random amplified polymorphic dna (rapd) and inter-simple sequence repeat (issr) markers were used to estimate genetic diversity and relationships among 35 cluster bean [cyamopsis tetragonoloba (l.) taub] genotypes. for rapd analysis, 20 random primers were used which amplified 164 bands, 147 of which were polymorphic with an average polymorphism of 90.3%. the amplified pro...

Spine gourd (Momordica dioica Roxb.) is a highly nutritious vegetable crop with dioecious reproductive nature. Forty-eight spine genotypes including 32 female and 16 male were assessed for molecular divergence to establish phenotypic relationships using ISSR markers. Twenty-two out of total 25 primers studied yielded 88 bands which 80 polymorphic, three them being unique in their profile. Each ...