An immunocapture reverse transcription polymerase chain reaction (IC-RT-PCR) method for a highly sensitive analysis of raspberry bushy dwarf virus (RBDV) in infected plants is described. In the method, preliminary purification of virus particles or viral RNA from the plant material is not necessary. Viruses are enriched during the assay by antibodies bound in the PCR microplate wells, followed ...

This review presents the basic principles of application loop-mediated isothermal amplification (LAMP) reaction for rapid diagnosis coronavirus infection caused by SARS-CoV-2. The technical details method, and most popular approaches specific non-specific detection products are briefly described. We also discuss first published works on use method nucleic acid SARS-CoV-2 virus, including those ...

The use of reverse transcriptase coupled with the polymerase chain reaction (RT-PCR) has been proven to be a valuable tool in the synthesis and isolation of cDNA clones as well as the quantification of rare messenger RNA species. While a number of different approaches can be used to permit gene-specific cDNA synthesis and amplification, a recurring problem is one of high background signal (i.e....

Due to the limitation of rapid development of specific antiviral drug or vaccine for novel emerging viruses, an accurate and rapid diagnosis is a key to manage the virus spread. We developed an efficient and rapid method with high specificity for the Middle East Respiratory Syndrome coronavirus (MERS-CoV), based on one-pot reverse transcription loop-mediated isothermal amplification (one-pot RT...

We evaluated the use of a newly described sodC-based real-time-polymerase chain reaction (RT-PCR) assay for detecting Neisseria meningitidis in normally sterile sites, such as cerebrospinal fluid and serum. The sodC-based RT-PCR assay has an advantage over ctrA for detecting nongroupable N. meningitidis isolates, which are commonly present in asymptomatic pharyngeal carriage. However, in our st...

Grass carp reovirus (GCRV) has been assigned to a newly established Aquareovirus genus in the family of Reoviridae which leads to haemorrhagic disease and extremely high mortality rate in grass carp. In this study, comparison was made between the novel one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) and the reverse transcription polymerase chain reaction (RT-PCR)...

A PCR based reverse transcriptase (RT) assay was developed that has 10(4)-fold higher sensitivity than conventional nucleotide incorporation assays and allows discrimination between false positive results generated by cellular polymerases and positives resulting from authentic RT activity. Recently, several reverse transcriptase (RT) assays have been developed where a reverse transcriptase reac...

The objective of this study is to compare and assess the effectiveness real-time polymerase chain reaction (RT-PCR), loop-mediated isothermal amplification (LAMP), selective agar plate method for detection Salmonella spp. Listeria monocytogenes in ready-to-eat (RTE) foods. In RTE foods, performance three methods (RT-PCR [SureTectTM kit PowerChekTM kit], LAMP [3M MDS], agar) were similar at 0-10...

INTRODUCTION The diagnosis of pleural tuberculosis requires an invasive and time-consuming reference method. Polymerase chain reaction (PCR) is rapid, but validation in pleural tuberculosis is still weak. OBJECTIVE To establish the operating characteristics of real-time polymerase chain reaction (RT-PCR) hybridization probes for the diagnosis of pleural tuberculosis. METHODS The validity of...

There is compelling support for widening the role of computed tomography (CT) COVID-19 in clinical and research scenarios. Reverse transcription polymerase chain reaction (RT-PCR) testing, gold standard diagnosis, has two potential weaknesses: delay obtaining results possibility RT-PCR test kits running out when demand spikes or being unavailable altogether. This perspective article discusses u...