Successful merging of chemical and biotechnological operations is essential to achieve cost-efficient industrialization of bio-based processes. The demonstration of the use of syngas, derived from microwave assisted pyrolysis of municipal solid waste, for the improved growth and poly-3-hydroxybutyrate production in Rhodospirillium rubrum, stands out as an example of the synergistic contribution...

Here, we report the genome sequence of Clostridium acetireducens (DSM 10703T), a strictly anaerobic bacterium capable of fermenting acetate and leucine to butyrate, isovalerate, and poly-3-hydroxybutyrate. The draft genome consists of a circular chromosome with a size of 2.4 Mb and harbors 2,239 predicted protein-encoding genes.

Sustainable production of bioplastics by heterotrophic microbes has been restricted by the limited resources of organic substrates and the energy required for biomass harvest. Here, the easy-to-harvest cyanobacterium (Chlorogloea fritschii TISTR 8527), from which the biomass instantaneously settled to the bottom of liquid culture, was utilized to produce poly-3-hydroxybutyrate (PHB) using a two...

Mitochondrial anaplerosis is important for insulin secretion, but only some of the products of anaplerosis are known. We discovered novel effects of mitochondrial metabolites on insulin release in INS-1 832/13 cells that suggested pathways to some of these products. Acetoacetate, beta-hydroxybutyrate, alpha-ketoisocaproate (KIC), and monomethyl succinate (MMS) alone did not stimulate insulin re...

Bioconversion of sugarcane molasses to poly(3-hydroxybutyrate-co-3- hydroxyvalerate) by endophytic Bacillus cereus RCL 02Rituparna Das, Arundhati Pal, Amal Kanti Paul

Ion-exclusion high-pressure liquid chromatography (HPLC) was used to measure poly-beta-hydroxybutyrate (PHB) in Rhizobium japonicum bacteroids. The products in the acid digest of PHB-containing material were fractionated by HPLC on Aminex HPX-87H ion-exclusion resin for organic acid analysis. Crotonic acid formed from PHB during acid digestion was detected by its intense absorbance at 210 nm. T...

Genes responsible for the synthesis of poly(3-hydroxybutyrate) (PHB) in Azotobacter sp. FA8 were cloned and analyzed. A PHB polymerase gene (phbC) was found downstream from genes coding for beta-ketothiolase (phbA) and acetoacetyl-coenzyme A reductase (phbB). A PHB synthase mutant was obtained by gene inactivation and used for genetic studies. The phbC gene from this strain was introduced into ...

Zoogloea ramigera 115 synthesized large amounts of matrix polymer from fructose, galactose, glucose, lactose, mannose, soluble starch, and sucrose when these carbohydrates were used as supplements to a chemically defined medium. All of them supported polymer synthesis to the extent that cultures thickened to a gel. Concentration of carbohydrate nutrients in the range 0.5 to 2.0% was not a criti...

Original Article Alcaligeneseutrophus H16, E. coli DH5α, operon phbCAB, Poly 3-Hydroxybutyrate (PHB).