A method to produce platelet-rich plasma and platelet concentrate using a double centrifuge technique in combination with the fibrin adhesive Tisseel is described. This technique constitutes the basic mixture for augmenting and improving an inadequate bone site. Also described is a procedure by which autologous bone or bone substitute is added to this mixture to increase the volume of grafting ...

The conventional method of assessing the platelet response to hypotonic stress (HSR) was adapted to allow microtitre plate technology to be used. After water is added to a platelet suspension two sequential readings are taken at 414 nM on a vertical microplate reader. The difference between the second (three minutes) and the first (one minute) was defined as the HSR. This method allowed the rel...

A 26-year-old male undergoing thoracotomy and bleeding control received a preoperative thoracic epidural for postoperative analgesia. On the fifth postoperative day, paralysis of both lower limbs occurred and urgent magnetic resonance imaging showed massive anterior epidural hematoma. During laminectomy and decompression, platelet dysfunction was diagnosed and preoperative non-steroidal anti-in...

Blood dilution after transfusion fluids leads to diminished coagulant activity monitored by rotational thromboelastometry, assessing elastic fibrin clot formation, or by thrombin generation testing. We aimed to determine the contributions of blood cells (platelets, red blood cells) and plasma factors (fibrinogen, prothrombin complex concentrate) to fibrin clot formation under conditions of haem...

according to limitations in blood product resources and to prevent unnecessary transfusions and afterwards complications in perioperative period of total hip arthroplasty, authors administered fibrinogen concentrate in a pilot randomized clinical trial to evaluate bleeding and need to blood transfusion in preoperative period. thirty patients (3-75 years old) with asa physical status class i or ...

Platelet-rich plasma (PRP) is obtained from a patient’s own peripheral blood, after centrifuging it, and has growth factors.[1] There are clinical studies indicating that PRP therapy may enhance muscle or tendon healing, and it accelerates the tissue regeneration after musculoskeletal injuries such as strain or contusion, during sports activities.[1,2] The application of these products is docum...

As use of autologous platelet-rich plasma (PRP) and platelet-poor plasma (PPP) increases for intraoperative care of a variety of patients, it is important for perioperative nurses to recognize their benefits. Autologous PRP may decrease postoperative drainage, reduce narcotic requirements, and facilitate an early return to mobility. Postoperatively, patients should experience fewer complication...

A 19-year-old woman with a qualitative variant of Glanzmann Thrombasthenia (GT) was referred to our department for her first pregnancy. The diagnosis of GT was revealed by epistaxis and gingival bleeding. Her history of bleeding was limited to dental procedures and haemorrhagic rupture of an ovarian cyst under platelet transfusion. Her husband had normal platelet function tests and no consangui...