A high-performance liquid chromatographic technique for the simultaneous determination of prednisolone and prednisone in human plasma, whole blood, urine, and bound-to-plasma proteins, using betamethasone as internal standard, is presented. Liquid-liquid extraction is used for whole blood samples, and solid phase extraction is used for plasma, urine, and proteins bound to plasma. The accuracy, ...

AgNPs-Tragacanth conjugate gel as an eco friendly and low cost membrane was applied for the effective electromembrane extraction (EME) of Tenofovir disproxil fumarate, an important acidic and high polar (log p=1.25) antiviral drug, followed by HPLC-UV determination. The effect of various parameters on the extraction efficiency including the presence of AgNPs, tragacanth gum concentration, gel t...

McNulty, Patrick H. Comparison of local and systemic effects of insulin on myocardial glucose extraction in ischemic heart disease. Am. J. Physiol. Heart Circ. Physiol. 278: H741–H747, 2000.—Physiological increases in circulating insulin level significantly increase myocardial glucose uptake in vivo. To what extent this represents a direct insulin action on the heart or results indirectly from ...

BACKGROUND Platelet-rich plasma (PRP) has been extensively used as a treatment in tissue healing in tendinopathy, muscle injury, and osteoarthritis. However, there is variation in methods of extraction, and this produces different types of PRP. PURPOSE To determine the composition of PRP obtained from 4 commercial separation kits, which would allow assessment of current classification systems...

An extractor has been developed for microporous membrane liquid-liquid extraction (MMLLE) of lipophilic xenobiotics at trace levels in biological fluids. This new construction allows the sample phase to be stirred, while the organic phase is pumped. The extractor was evaluated using human blood plasma with added organophosphate esters. The size exclusion properties of the membrane reduced lipid...

Lipid extraction from biological samples is a critical and often tedious preanalytical step in lipid research. Primarily on the basis of automation criteria, we have developed the BUME method, a novel chloroform-free total lipid extraction method for blood plasma compatible with standard 96-well robots. In only 60 min, 96 samples can be automatically extracted with lipid profiles of commonly an...

BACKGROUND Knowledge of the fatty acid composition of lipid classes in human plasma is an important factor in the investigation of human metabolism. Therefore, a method for the analysis of neutral lipid (NL), phospholipid (PL) and free fatty acids (FFA) in human plasma has been developed and validated. METHODS Separation of lipid classes was carried out by solid phase extraction of the lipid ...

aim and objectives: the hepatitis c virus (hcv) is essentially hepatotropic but virus compartment have also been found in other important extra hepatic sites. detection of hcv rna in extra hepatic reservoirs such as peripheral blood mononuclear cells (pbmcs) is important for determining of disease progression and effective treatment. the present study aimed to determining of different hcv genot...