Previous measurements in lymph and adipose tissue have indicated that interstitial insulin concentrations are ∼40% lower than in plasma. Measurements of insulin in human muscle interstitial fluid have not been performed yet. We developed a new external reference technique for calibration of microdialysis catheters in situ. This technique allows correct assessments of interstitial peptide concen...

background and objectives this study was undertaken to evaluate the effect of the cultivated satureja khuzestanica essential oil (skeo) on activity and gene expression of hepatic gk in normal and diabetic rats.   methods biochemical parameters such as glucose, insulin, and hba1c plasma levels and oral glucose tolerance test were all analyzed. the cultivated skeo was orally administered at diffe...

The characteristics of plasma free insulin profiles after conventional subcutaneous injection of regular insulin (10 units) and after jet injection of this amount of insulin were compared in eight subjects with insulin-dependent diabetes mellitus. Although administration of insulin with the jet injector resulted in peak plasma free insulin concentrations (45 +/- 4 microU/ml) similar to those ac...

Although the lens has been shown to have the capacity to respond to insulin in vitro, little is known concerning the biochemical relationships of insulin to the lens in vivo. Therefore we have measured insulin in the aqueous humor of rabbits by a sensitive radioimmunoassay after paracentesis and feeding. The insulin concentration in aqueous humor was 3% of that in plasma. One hour after paracen...

We have investigated the relationships between plasma interleukin-6 (IL-6) levels and insulin sensitivity and insulin secretion in a cohort of Italian-Caucasian glucose-tolerant subjects. Insulin sensitivity was assessed by euglycemic-hyperinsulinemic clamp, and first-phase insulin secretion was measured by intravenous glucose tolerance test. Fasting plasma IL-6 concentration was negatively cor...

Aim: To assess insulin secretion/insulin resistance index in predicting the risk for future T2DM Experimental Design and Methods: 1551 non-diabetic subjects from the San Antonio Heart Study received an OGTT with measurement of plasma glucose and insulin concentrations at 0, 30, 60, and 120 minutes at baseline and after 7-8 years of follow-up. Insulin secretion/insulin resistance index was calcu...

introduction: many of the diseases of adulthood are originated from the intrauterine conditions during fetal life. because of the importance of thyroid hormones in growth and development of the fetus, the effects of maternal hypothyroidism on carbohydrate metabolism in adulthood was investigated. methods: pregnant rats were divided into the fetal hypothyroidism (fh) and the control (c) groups. ...

The concentration of tryptophan in rat doses of glucagon (1 mg/kg) reduce plasma increases by 30-40/0o 2 hr after plasma tryptophan concentrations. fasting animals receive insulin (2 U/kg) Lower doses are without a significant or consume a carbohydrate meal. effect. The sources of plasma trypto-These treatments decrease the con-phan responsible for its increase after centrations of all other am...

In normal fasted rats whole liver tissue contains as much glucose as the blood plasma, i.e., the ratio of the concentrations is about unity. The concentration of glucose in hepatic intracellular water is about 1.2 times higher than in plasma water. In rats injected with insulin the concentration of glucose in the liver falls to a lesser extent than in the plasma: resulting in a ratio of concent...