leaf and root explants from 10 to 20 day– old seedlings of plantago lanceolata, were cultured on ms, msh, nt and b5 basal media with various concentrations of different plant growth regulators. the best callus induction was obtained with 0.4 mgl-1 2,4-d in msh i with 400 callus index. callus growth was significantly stimulated (p=0.05)with 0.8 mgl-1 2,4-d and 0.1 mgl-1 kin in msh ii, with 7...

abstract gerbera is one of the most demanded plants in commercial cut flowers plants. optimizing the micro-propagation of new cultivars is therefore important. in this research “red explosion” cultivar was used and capitola (as explants) were directed to a modified solid murashigue and skoog medium with four concentration of tdz (0.25, 0.50, 0.75 and 1.00 mgl-1) for regeneration. three concentr...

Nanoparticles influence on genome is investigated worldwide. The appearance of somaclonal variation is a cause of great concern for any micropropagation system. Somaclonal variation describes the tissue-culture-induced phenotypic and genotypic variations. This paper shows the results of somaclonal variation in two resistance genes pectin methylesterase and Mlo-like protein in all tissue culture...

R. García-Gonzáles, K. Quiroz, B. Carrasco, and P.D.S. Caligari. 2010. Plant tissue culture: Current status, opportunities and challenges. Cien. Inv. Agr. 37(3): 5-30. In the last two decades plant biotechnology applications have been widely developed and incorporated into the agricultural systems of many countries worldwide. Tissue culture tools have been a key factor to support such outcomes....

The problem of antibiotic resistance emerging from the use and misuse of antibiotics in veterinary practice was considered as long ago as the 1960s by the famous Swann Committee. 1 What seems to be less well understood, and certainly less well documented, is the use of antimicrobials in the world of plant science. Antibiotics have been widely used in the control of contamination of micropropaga...

Plant biotechnology has emerged as a powerful tool of crop improvement and has aroused a great deal of interest in many countries because of its application to agriculture and horticulture. We report here the application of a number of in vitro plant breeding techniques such as micropropagation, in vitro flowering, in vitro pollination and fertilisation, anther culture, somatic embryogenesis, a...