This study examined the potential utility of DNA sequence analysis to discriminate and identify plant material in forensic investigations. DNA was extracted from plant leaf fragments of 11 species stored for 5 to 22 years after collection. The trnH-psbA intergenic spacer and 316 bp of the rbcL gene were successfully amplified and sequenced for all fragments except for the trnH-psbA spacer of on...

This study was undertaken to determine the concentrations of carbofuran residues in water, soil and plant samples from selected sites in the farmlands in Kenya and to demonstrate the impact of Furadan use on the local environment. Soil, water and plant samples obtained from agricultural farmlands where the technical formulation Furadan has been used extensively showed high environmental contami...

A robust multi-residue procedure is needed for the analysis of the pro-herbicide isoxaflutole and its degradates in soil and plant materials at environmentally relevant (<1 microg kg-1) levels. An analytical method using turbo-spray and heat-nebulizer high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed for the analysis of isoxaflutole (IXF) and its two met...

We evaluated exposure of aquatic biota to lead (Pb), zinc (Zn), and cadmium (Cd) in streams draining a Pb-mining district in southeast Missouri. Samples of plant biomass (detritus, periphyton, and filamentous algae), invertebrates (snails, crayfish, and riffle benthos), and two taxa of fish were collected from seven sites closest to mining areas (mining sites), four sites further downstream fro...

A PCR-based diagnostic assay was developed for early detection and identification of Aphelenchoides fragariae directly in host plant tissues using the species-specific primers AFragFl and AFragRl that amplify a 169-bp fragment in the internal transcribed spacer (ITS1) region of ribosomal DNA. These species-specific primers did not amplify DNA from Aphelenchoides besseyi or Aphelenchoides ritzem...

PREMISE OF THE STUDY An efficient, effective DNA extraction method is necessary for comprehensive analysis of plant genomes. This study analyzed the quality of DNA obtained using paper FTA cards prepared directly in the field when compared to the more traditional cetyltrimethylammonium bromide (CTAB)-based extraction methods from silica-dried samples. METHODS DNA was extracted using FTA cards...

abstract in this study, two wild iranian crocus species (crocus speciosus and crocus cancellathus) were examined using 16 morphological characters and 13 issr primers on 250 and 25 plant samples, respectively. the highest and lowest coefficient of variations were belonged to the thickness of the petals (71.25%) and the leaf length (15.08%), respectively. cluster analysis based on morphological ...

The diversity of viruses associated with non-cultivated plants was assessed from plant samples collected in the Tallgrass Prairie Preserve of northeastern Oklahoma, USA. The samples were processed to determine the sequences of nucleic acids extracted from the virus-like particle fraction of plant homogenates. Sequences from 95 specimens of 52 plant species included those of probable origin from...

Plant organs are composed of many different cell types and the analysis of 'bulk' material results in the average of all information in these cells. Therefore, this does not reflect any individuality of the tissues present in plants. This review briefly summarizes different sampling methods which provide tissue- and cell-specific samples, respectively. In addition, gene expression analysis tool...

Water hyacinths are aquatic plants that indicate a capability for pollutants uptake. Recently, further efforts have been made to increase the capacity of the plant to remediate the pollutants. Water hyacinth roots and shoots were analyzed using FTIR spectroscopy, XRF, and electrical investigations. Results of XRF analyses indicate that except for Sr, CaO, Na2O, and K2O, the level of metals as w...