Pichia pastoris is commonly used to express and secrete target proteins, although not all recombinant proteins can be successfully produced. In this study, we used methyl parathion hydrolase (MPH) from Ochrobactrum sp. M231 as a model to study the importance of the N-terminus of the protein for its secretion. While MPH can be efficiently expressed intracellularly in P. pastoris, it is not secre...

This research utilized the Pichia pastoris expression system for recombinant expression of cDNA of pleurocidin, a small (2.7 kd) antimicrobial peptide isolated from winter flounder (Pleuronectes americanus). The Pichia vector contains the alcohol oxidase gene promoter (AOX 1), which under the induction of methanol allows for expression of heterologous protein gene inserted downstream in the vec...

The six strains of the Pichia species, once classified in the genus Petasospora, were examined for their 18S (positions 1451-1618, 168 bases) and 26S (positions 1611-1835, 225 bases and 493-622, 130 bases) rRNA partial base sequencings. In the 18S rRNA partial base sequencings, the type species of the genus Petasospora (Petasospora rhodanensis) was found to be closely related to Pichia anomala ...

In a field experiment the susceptibility of yeast-like fungi to selected fungicides with the active ingredients (a.i.) propiconazole, epoxyconazole, kresoxim-methyl, fenpropimorph, carbendazim, prochloraz and flusilazole, was analyzed. The direct effect of the fungicides on the colonies of yeast-like fungi was determined based on antibiograms – the diffusion method. The survival rate of the iso...

Xylanase, which catalyzes the random hydrolysis of internal xylosidic linkages, is a critical enzyme participating in xylan decomposition and has been widely applied in industrial utilizations. Xylanase isolated from the extremophilic Streptomyces sp. S9 (XynAS9) possesses broad adaptability to temperature and pH and thus is an attractive candidate in industrial applications. In particular, the...

objectives: in present research we evaluate the expression of this critical enzyme in a eukaryotic system for future use in cheese industry. materials and methods: we have cloned bovine prochymosin gene in methylotrophic yeast, p. pastoris, using ppic9k as an expression vector. the recombinant plasmid was transformed into the host by electroporation, and it was expressed in optimum con­ditions...

The new methanol-assimilating yeast species Komagataella phaffii Kurtzman sp. nov. (type strain NRRL Y-7556(T)=CBS 2612(T)) is described. Of the four known strains of this species, two were isolated from black oak trees in California, USA, one from an Emory oak in Arizona, USA, and one from an unidentified source in Mexico. The species forms hat-shaped ascospores in deliquescent asci and appear...

Measurement of Size Distributions of Bacterial Cells. R. J. HARVEY AND ALLEN G. MARR.................................................................. 805 Paper Chromatographic System for the Identification of Glycerol in Bacterial Cell Walls. MIYOSHI IKAWA, JAMES W. MORROW, AND SHEILA J. HARNEY........... 812 Desulfovibrio africanus sp. n., a New Dissimilatory Sulfate-reducing Bacterium. L. LE...

A synthetic method was developed to chemically attach few molecules of simple sugars like glucose, mannose, galactose, maltose and xylose (0.09 0.37 wt%) and with quaternary nitrogen pendants (0.42 0.46 atomic%) along the polybutadiene section of polystyrene-block-polybutadiene-block-polystyrene (SBS) block copolymer. These functionalized SBS copolymers were evaluated for biodegradation using t...

Malaria causes high global mortality and morbidity annually. Plasmodium knowlesi has been recognised as the fifth human Plasmodium sp. and its infection is widely distributed in Southeast Asia. Merozoite surface protein-119 (MSP-119) appears as a potential candidate for malaria blood stage vaccine as it could induce protective immunity. In this study, codon optimized P. knowlesi MSP-119 (pkMSP-...