Mechanisms by which lymphocytes stimulate glycosaminoglycans (GAG) production and glucose utilization by cultures of retrobulbar fibroblasts were investigated. Lymphocytes must be living or undergo at least one day of preincubation before freeze-thawihg to be active. Preliminary exposure of lymphocytes to phytohemagglutinin for one day inconsistently induced small increases in the lymphocyte po...

By JEROME I. BRODY AND HENRY D. SOLTYS T HE PUBPOSE of this study was to delineate the durability and persistence of immunologic recall in the normal peripheral blood lymphocyte using methods which might reflect the importance of the manner by which imniunologic information is acquired. This was done in the present investigation with the technic of bacterial adherence,”2 in which circumferentia...

The DNA-dependent RNA polymerase activities of isolated nuclei from lymphocytes were examined after stimulation with phytohemagglutinin (PHA). The nuclear fraction was prepared with Mg(++) or Mn(++) to distinguish between polymerase I (nucleolar) and polymerase II (nucleoplasmic). Distinction between polymerases II and III was obtained by the addition of alpha-amanitin to the reaction mixture. ...