TagRFP, a fluorescent protein capable of photosensitizing the production of singlet oxygen, was expressed in E. coli. Subsequent exposure to green light induced bacterial cell death in a light-dose dependent manner. It is demonstrated for the first time that intracellular singlet oxygen is sufficient to kill bacteria.

To obtain some information of the biological action of Kankohso 101dinicotinate and Kankohso 301-nicotinate, observations were made on the binding mode of these substances with protein, chondroitin sulfate and nucleic acids and the following results were obtained; 1. Kankohso 10 I-dinicotinate binds reversively with bovine serum albumin or serum r-globulin, resulting in metachromasia. By bindin...

Photodermatoses are a group of disorders resulting from abnormal cutaneous reactions to solar radiation. They include idiopathic photosensitive disorders, drug or chemical induced photosensitivity reactions, DNA repair-deficiency photodermatoses and photoaggravated dermatoses. The pathophysiology differs in these disorders but photoprotection is the most integral part of their management. Photo...

The capacity of various furocoumarins to generate singlet oxygen in aqueous solution has been determined. The antiproliferative and the skin-photosensitizing activities of the same furocoumarins did not show any correlation with the capacity to generate the singlet oxygen, while these photobiological properties could be correlated with the capacity of furocoumarins to induce photolesions to the...

Photosensitizing flavoproteins have great potential as tags for correlative light and electron microscopy (CLEM). We examine the photostability of miniSOG mutants and their ability to photo-oxidize diaminobenzidine, both key aspects for CLEM. Our experiments reveal a complex relation between these parameters and the production of different reactive oxygen species.

π-Conjugated microporous polyHIPEs are synthesized via Suzuki cross-coupling. The polymeric backbone consists of electron donor and acceptor moieties with suitable energetic characteristics to promote the efficient photosensitizing ability for the activation of singlet oxygen, showing extraordinarily high stability. The reaction can be carried out in a continuous flow reaction set-up, realizing...

The main aim of the present work was to investigate the potential effect of acetone extract of Ficus religosa leaf (FAE) in multiple apoptosis signalling in human breast cancer cells. FAE treatment significantly induced dose and time dependent, irreversible inhibition of breast cancer cell growth with moderate toxicity to normal breast epithelial cells. This observation was validated using Sulf...

Defective repair of sunlight-induced DNA photodamage, coupled with an unusually high occurrence of multiple primary basal cell carcinomas (BCCs), is the major characteristic of xeroderma pigmentosum. Our recent work has indicated that this etiological paradigm may apply to skin cancer patients without an apparent hereditary disease. The present study reports on an investigation of whether medic...

Reaction of porphycene isothiocyanates with primary and secondary amines leads to the formation of thiazolo[4,5-c]porphycenes, with a substantial shift in the absorption and fluorescence spectra. The conjugates show fluorescence in the near-infrared and are capable of photosensitizing the production of the cytotoxic species singlet oxygen.