SUMMARY: A simple glass slide technique has been devised for the continuous microscopical observation of growth and infection of root hairs of clover seedlings. The method involves an aseptic cultivation of seedlings on microscope slides which are partly immersed in a mineral salts medium. The roots are protected by a cover-slip. By this procedure, the root hairs of white clover inoculated with...

Borrelia burgdorferi is a motile spirochete with multiple internal periplasmic flagella (PFs) attached near each end of the cell cylinder; these PFs overlap in the cell center. We analyzed the shape and motion of wild type and PF-deficient mutants using both photomicrography and video microscopy. We found that swimming cells resembled the dynamic movements of eukaryotic flagella. In contrast to...

A series of experiments have been performed to determine the mechanisms of failure in compressively loaded laminated plates in the presence of stress gradients generated by a circular cutout. Real time holographic interferometry and in situ photomicrography of the hole surface along with strain gauge measurements are used to study the progression of failure. The test specimens are multilayered ...

The effect of living hemolytic streptococci on ascites tumor cells (Sarcoma 37 and Krebs-2 carcinoma) was studied in vitro by phase microscopy and in vivo by implanta tion into mice. The effects produced by Streptococcus pyogenes strains were compared with those of Serraiia marcescena, Sareina lutea, and Streptococcuafuecalis. Three of the eight streptococcal strains were highly effective again...

Photomicrography and reflectance microphotometry were used to monitor melanosome movement in frog skin melanocytes in vitro in response to hormonal stimulation and cytochalasin B (CB). Melanocyte-stimulating hormone (MSH), theophylline, and dibutyryl cyclic AMP (DiBcAMP) induced melanosome dispersion (darkening) which was promptly arrested by cytochalasin B in concentrations of 5-20 microg/ml. ...

A marine-derived red alga, along with their active constituents, were actively screened for biocontrol potential against major insect pests. The current study explored the larvicidal activity of crude methanolic extracts alga Halymenia dilatata (Mx-Hd) Aedes aegypti (Linn.), and enzyme inhibition, repellent activity, non-target toxicity was species. Mx-Hd to exposed larvae dose-dependent, it si...

The objective of this paper is to describe a new approach for locating the centromere of each chromosome displayed in the digitalized photomicrography of fish cells. To detect the centromere position, the authors propose methods for both image segmentation and split touching chromosomes based on the fuzzy sets theory and a method for the rotation of chromosomes. These methods were applied to tw...