In the course of synthesizing a number of aryl phosphate esters as substrates for prostatic acid phosphatase, polymeric phosphorylated products obtained by reaction of 4,4’-dihydroxybiphenyl with phosphorus oxychloride were found to be potent enzyme inhibitors. This report describes the preparation of these anionic polymers and some characteristics of their inhibition of prostatic acid phosphat...

ortho-Lithiation of cyclic aryl sulfonamides in the presence of phosphoryl chloride provides a very simple entry to fused polycyclic sultams (benzothiazolines and naphthathiazolines).

Glycogen phosphorylase has been isolated from rabbit muscle in two different molecular forms, phosphorylase a, a tetramer with a molecular weight of 495,000 (2-4), and phosphorylase b, a dimer with a molecular weight of 242,000 (3-5). The dimerization of the protein that occurs in the conversion of phosphorylase 6 to a with phosphorylase kinase, Mg++, and ATP has been found to result from the i...

We have probed the electrostatic environment of the active site of the Tetrahymena group I ribozyme (E) using protonated 2'-aminoguanosine (), in which the 2'-OH of the guanosine nucleophile (G) is replaced by an group. At low concentrations of divalent metal ion (2 mM Mg(2+)), binds at least 200-fold stronger than G or G(NH)()2, with a dissociation constant of </=1 microM from the ribozyme. ol...

Equilibration of the phosphorus in radioactive phosphoric acid with the phosphorus in phosphorus oxychloride occurs if these compounds are refluxed together for approximately 24 h. This observation led us to develop a method for the preparation of radioactive phosphorus oxychloride on a small scale with high specific radioactivity. The labeled phosphorus oxychloride may be utilized directly in ...

2- Ethoxy-4(3H) quinazolinone 1 was synthesized and allowed to react with various halides, namely: alkyl, benzyl, allyl, acyl, haloacetyl, crotonyl, benzoyl, 2-furoyl and 1-naphthalenesulphonyl halides affording quinazoline and quinazolinone derivatives. The reactions of compound 1 with phosphorus oxychloride, phosphorus pentasulfide, ethyl chloroformate, ethyl chloroacetate, ?-bromoglucose tet...

The active site of the cAMP-dependent protein kinase catalytic subunit harbors a cluster of acidic residues-Asp 127, Glu 170, Glu 203, Glu 230, and Asp 241-that are not conserved throughout the protein kinase family. Based on crystal structures of the catalytic subunit, these amino acids are removed from the site of phosphoryl transfer and are implicated in substrate recognition. Glu 230, the m...