Simple sulfonamide and amide derivatives of tris(2-aminoethyl)amine (Tren) are known to promote the translocation or flip-flop of phosphatidylcholine, but not phosphatidylserine, across bilayer membranes. This paper describes the synthesis of a 300-member, spatially encoded library of Tren derivatives with appended peptide--sulfonamide and peptide--urea arms. The library was synthesized using t...

Platelets are known to participate in all phases of haemostasis in vivo. Besides the obvious contribution in formation of the platelet plug, they provide the surface necessary for the assembly of the tenase and prothrombinase complexes, a process leading to the formation of thrombin, and ultimately the fibrin clot. The complex assembly involves the translocation of negatively charged phosphatid...

P4-ATPases comprise a relatively new subfamily of P-type ATPases implicated in the energy dependent translocation of aminophospholipids across cell membranes. In this study we report on the localization and functional properties of Atp8a2, a member of the P4-ATPpase subfamily that has not been studied previously. RT-PCR revealed high expression of Atp8a2 mRNA in the retina and testis. Within th...

The Bacillus subtilis pss gene encoding phosphatidylserine synthase was cloned by its complementation of the temperature sensitivity of an Escherichia coli pssA1 mutant. Nucleotide sequencing of the clone indicated that the pss gene encodes a polypeptide of 177 amino acid residues (deduced molecular weight of 19,613). This value agreed with the molecular weight of approximately 18,000 observed ...

Apoptotic cells expose phosphatidylserine on their surface as an "eat me" signal, and macrophages respond by engulfing them. Although several molecules that specifically bind phosphatidylserine have been identified, the molecular mechanism that triggers engulfment remains elusive. Here, using a mouse pro-B cell line, Ba/F3, that grows in suspension, we reconstituted the engulfment of apoptotic ...

P(4)-ATPases comprise a relatively new subfamily of P-type ATPases implicated in the energy-dependent translocation of aminophospholipids across cell membranes. In this study, we report on the localization and functional properties of Atp8a2, a member of the P(4)-ATPase subfamily that has not been studied previously. Reverse transcription-PCR revealed high expression of atp8a2 mRNA in the retin...