Abstract Phenolic acids are the major secondary metabolites and significant bioactive constituents of medicinal plant Salvia miltiorrhiza. Many enzyme-encoding genes transcription factors involved in biosynthesis phenolic have been identified, but underlying post-translational regulatory mechanisms poorly understood. Here, we demonstrate that S. miltiorrhiza Kelch repeat F-box protein SmKFB5 ph...

Banana blossoms are rich in fiber and nutrients a popular plant-based, vegan alternative to fish. However undesirable browning, usually visible at the peduncle cut-end, negatively impacts consumer acceptability of banana blossoms. The aim this work was develop safe alternatives prevent browning First, activities primary enzymes associated with tissue i.e., polyphenol oxidase (PPO), peroxidase (...

Abstract Nitric oxide (NO) is a highly reactive gaseous small molecule that regulates plant growth. Sodium nitroprusside (SNP) was used as an NO donor to study the effects of treatment on browning and antioxidant activity button mushrooms ( Agaricus bisporus ). The findings revealed exogenous alleviated browning, decreased hardness, increased weight loss stored at 4°C for 15 days, reduced accum...

Large and rapid increases in the activities of two enzymes of general phenylpropanoid metabolism, phenylalanine ammonia-lyase and 4-coumarate:CoA ligase, occurred in suspension-cultured parsley cells (Petroselinum hortense) treated with an elicitor preparation from Phytophthora megasperma var. sojae. Highest enzyme activities were obtained with an elicitor concentration similar to that required...

In recent years, the application of natural substances on crops has been intensified in order to increase the resistance and yield of the soybean crop. Among these products are included plant biostimulants that may contain algae extracts, amino acids, and plant regulators in their composition. However, there is little information on the isolated effect of each of these constituents. The objecti...

Compounds with planar triple ring systems such as acridine orange, 9-amino acridine, 9-amino-1,2,3, 4-tetrahydroacridine (tacrine), 6,9-diamino-2-ethoxyacridine lactate monohydrate (DE-acridine), 6-chloro-9-(3'-diethylamino-2'-hydroxypropylamino)-2-methoxyacridine 2 HCI (CDM-acridine), quinacrine, 6-chloro-9(4'diethylamino1'-methylbutylamino) .2-methoxy. 1, 10-diazaanthracene (CDM 1, 10-diazaan...

A heterologous probe encoding phenylalanine ammonia-lyase (PAL) was used to identify PAL clones in cDNA libraries made with RNA from young leaf tissue of two Populus deltoides x P. trichocarpa F1 hybrid clones. Sequence analysis of a 2.4-kb cDNA confirmed its identity as a full-length PAl clone. The predicted amino acid sequence is conserved in comparison with that of PAL genes from several oth...

Novel hybrid magnetic cross-linked enzyme aggregates of phenylalanine ammonia lyase (HM-PAL-CLEAs) were developed by co-aggregation of enzyme aggregates with magnetite nanoparticles and subsequent crosslinking with glutaraldehyde. The HM-PAL-CLEAs can be easily separated from the reaction mixture by using an external magnetic field. Analysis by scanning electron microscopy (SEM) and confocal la...

Signal transduction via phospholipids is mediated by phospholipases such as phospholipase C (PLC) and D (PLD), which catalyze hydrolysis of plasma membrane structural phospholipids. Phospholipid signaling is also involved in plant responses to phytohormones such as salicylic acid (SA). The relationships between phospholipid signaling, SA, and secondary metabolism are not fully understood. Using...

Salicylic acid (SA) is an elicitor to induce the biosynthesis of secondary metabolites in plant cells. Hydrogen peroxide (H2O2) plays an important role as a key signaling molecule in response to various stimuli and is involved in the accumulation of secondary metabolites. However, the relationship between them is unclear and their synergetic functions on accumulation of secondary metabolites ar...