A new phenotypic test, called the Carbapenem Inactivation Method (CIM), was developed to detect carbapenemase activity in Gram-negative rods within eight hours. This method showed high concordance with results obtained by PCR to detect genes coding for the carbapenemases KPC, NDM, OXA-48, VIM, IMP and OXA-23. It allows reliable detection of carbapenemase activity encoded by various genes in spe...

data related to holstein dairy cattle from 1987 to 2004 were obtained from animal breeding center of iran to estimate the genetic and phenotypic trends. variance components and genetic parameters of the first lactation records were estimated using restricted maximum likelihood method and aireml algorithm through matvec program. animal breeding values were predicted through multiple-trait animal...

in this study, the number of 1802 longevity records of ewes collected during 1989 to 2010 in breeding station of lori-bakhtiari sheep flocks were used to estimate of genetic, environmental and phenotypic trends for economic lifetime. the breeding values of a ewe’s lifetime were estimated by restricted maximum likelihood procedure under an animal model of univariate analysis. the best linear unb...

High-throughput image-based screening (also termed high-content screening) has become a popular method in systems biology, functional genomics and drug discovery. Data analysis of high-content screening can be divided into two steps: image quantification and phenotypic analysis. Previously we have developed two R packages, EBImage and imageHTS for image quantification. The current R package phe...

Novelty search is an algorithm which proposes open-ended exploration of the search space by maximising behavioural novelty, removing the need for an objective fitness function. However, we show that when applied to complex tasks, training through novelty alone is not sufficient to produce useful controllers. Alongside this, the definition of phenotypic behaviour significantly effects the strate...

Background & Aims: Organisms producing TEM-β-lactamase are emerging around the world as a source of resistance to β-lactam antibiotics such as three generation cephalosporins. In this study, we used a polymerase chain reaction (PCR) to identify genes TEM for Extended-spectrum β-lactamase (ESBLs) producing clinical isolates of Klebsiella pneumonia from hospitals of Boro...

Contamination sites of Pseudomonas fluorescenswere traced in the production line for milk pasteurization in a large dairy plant in Shiraz, Iran. Samples of raw and pasteurized milk were collected at six sites along the line. All milk samples were incubated at 7°C until the aerobic plate count had reached 10 6 -10 7 cfu mL-1. Colonies were picked randomly and identified. No growth of gram negati...

BACKGROUND Methicillin-resistant Staphylococcus aureus (MRSA) is the main cause of hospital infection emerged over the last decades. Rapid detection of MRSA is important for patient care and proper usage of infection control. Detection of mecA genes (encoding resistance to methicillin and other similar antibiotics) and nuc genes (encoding staphylococcal thermostable nuclease) by PCR method is n...