conclusions the findings of the current study indicate that at high concentrations of salt, the growth of bacteria reduces so that it stops at a concentration of 50 mg l-1 (5%). thus, the bacterium is halotolerant or halophilic. with an increase in phenol concentration, the growth rate increased. phenol toxicity appears at a concentration of 600 mg l-1. results the phenol concentration of 600 m...

Several floc-forming bacteria and phenol-decomposing bacteria were isolated from a phenol-adapted activated sludge. Strain No. 12 was the best floc-former and the ratio of flocculated cells to total cells was 90 or more. A model floc was formed by the mixed culture of the strain No. 12 and No. 3, which was one of representative strains of phenoldecomposing bacteria. This model floc can successf...

DNA was isolated from phenol-digesting activated sludge, and partial fragments of the 16S ribosomal DNA (rDNA) and the gene encoding the largest subunit of multicomponent phenol hydroxylase (LmPH) were amplified by PCR. An analysis of the amplified fragments by temperature gradient gel electrophoresis (TGGE) demonstrated that two major 16S rDNA bands (bands R2 and R3) and two major LmPH gene ba...

A method for quantifying bacterial populations introduced into an activated-sludge microbial community is described. The method involves extraction of DNA from activated sludge, appropriate dilution of the extracted DNA with DNA extracted from nonintroduced activated sludge, PCR amplification of a gyrB gene fragment from the introduced strain with a set of strain-specific primers, and quantific...

background: the contamination of ecosystem with petroleum and its derivatives is con­sidered as one of the most crucial environmental threat in iran. application of micro­organisms has been demonstrated as an appropriate and more practical alternative to clean-up petroleum hydrocarbons in the contaminated environments. objectives: the objectives of this study were isolating rhizosphere-inhabiti...

The phenol-degrading strain Trichosporon cutaneum R57 utilizes various aromatic and aliphatic compounds as a sole carbon and energy source. The intracellular activities of phenol hydroxylase [EC 1.14.13.7] of a Trichosporon cutaneum R57 strain grown on phenol (0.5 g/l) were measured. Different toxic phenol derivatives (cresols, nitrophenols and hydroxyphenols) were used as substrates in the rea...

The coking process produces great volumes of wastewater contaminated with pollutants such as cyanides, sulfides and phenolics. Chemical and physical remediation of this wastewater removes the majority of these pollutants; however, these processes do not remove phenol and thiocyanate. The removal of these compounds has been effected during bioremediation with activated sludge containing a comple...