The manganese peroxidase (MnP) of Phanerochaete chrysosporium supported Mn(II)-dependent, H(2)O(2)-independent lipid peroxidation, as shown by two findings: linolenic acid was peroxidized to give products that reacted with thiobarbituric acid, and linoleic acid was peroxidized to give hexanal. MnP also supported the slow oxidation of phenanthrene to 2,2'-diphenic acid in a reaction that require...

Environmental contaminants, such as polycyclic aromatic hydrocarbons (PAHs), can disrupt the endocrine system and affect reproductive function of humans and wildlife. In this study, we exposed Japanese medaka (Oryzias latipes) to water-borne or food-spiked phenanthrene, an ubiquitous PAH, and investigated the chronic effects of the chemical on medaka reproduction. The results showed that phenan...

In this study, oxic and anoxic microcosms were constructed with aquifer sediment collected from the vicinity of a municipal solid waste composting site.Phenanthrene was used as a model PAH compound. The changes of bacterial and archaeal communities in microcosms were characterized with terminal restriction fragment length polymorphism. 16S rRNA gene clone library analysis was also used to inves...

Acidovorax sp. strain NA3 was isolated from polycyclic aromatic hydrocarbon (PAH)-contaminated soil that had been treated in a bioreactor and enriched with phenanthrene. The 16S rRNA gene of the isolate possessed 99.8 to 99.9% similarity to the dominant sequences recovered during a previous stable-isotope probing experiment with [U-(13)C]phenanthrene on the same soil (D. R. Singleton, S. N. Pow...

We carried out molecular orbital theory calculations for the homogeneous gas-phase formation of dibenzofuran from phenanthrene, fluorene, 9-methylfluorene and 9-fluorenone. Dibenzofuran will be formed if ∙OH adds to C8a, and the order of reactivity follows as 9-fluorenone > 9-methylfluorene > fluorene > phenanthrene. The oxidations initiated by ClO∙ are more favorable processes, considering tha...

Polyporus sp. S133, a fungus collected from contaminated-soil, was used to degrade phenanthrene, a polycyclic aromatic hydrocarbon (PAH), in soil. A maximal degradation rate (89%) was obtained when Polyporus sp. S133 was cultured with shitake’s nutrient addition for 30 days, as compared to just 44% degradation in non-supplemented cultures. When the fungus Polyporus sp. S133 was grown in presenc...

The objective of this study was to investigate early biological response in olive flounder exposed to sub-lethal concentrations of waterborne phenanthrene (0.5, 1 or 2 microM). The fish were exposed for 4 weeks and we analyzed their enzymatic defense system, antioxidant and phase II enzyme activities, to evaluate the chronic exposure toxicity of phenanthrene. Waterborne phenanthrene affected an...

A study was undertaken to assess if corn plant (Zea may L.) maybe able to enhance the degradation of phenanthrene and pyrene in acidic soil inoculated with a bacterial strain (Pseudomonas putida MUB1) capable of degrading polycyclic aromatic hydrocarbons (PAHs). Planting with corn, inoculating with MUB1, ora combination of the two were found to promote the degradation of phenanthrene and pyrene...

In a previous study, we showed that the halophyte plant model Thellungiella salsuginea was more tolerant to phenanthrene (Polycyclic Aromatic Hydrocarbon: PAH) than its relative glycophyte Arabidopsis thaliana. In the present work, we investigated the potential of another halophyte with higher biomass production, Cakile maritma, to reduce phenanthrene phytotoxicity. Sand was used instead of ara...

Mycobacterium sp. strain AP1 grew with pyrene as a sole source of carbon and energy. The identification of metabolites accumulating during growth suggests that this strain initiates its attack on pyrene by either monooxygenation or dioxygenation at its C-4, C-5 positions to give trans- or cis-4,5-dihydroxy-4,5-dihydropyrene, respectively. Dehydrogenation of the latter, ortho cleavage of the res...