Abstract In this study, a strong anionic resin was used to remove the chloride ion in diethanolamine of sweetening unit gas refinery. A laboratory pilot has been investigated as resin-filled bed evaluate reaction temperature, concentration, saturation, recovery rate, optimal flow hydrogen strength and performance work. The saturation time, capacity resin, temperature changes concentration outpu...

The behavior of ion-exchange chromatography is well understood with respect to changes in ionic strength, pH, resin ligand density, bed height, elution flow rate, and gradient slope. Their relative importance for any specific chromatographic situation varies. When a chromatographic operation utilized to purify a human therapeutic protein is prepared for validation before commercial production, ...

The present paper deals with the synthesis, ion exchange properties and analytical applications of basic lead molybdate as a new inorganic ion-exchanger. A simplified synthesis and physicochemical properties which show that the chelating resin HQCR4 in H behaves as a weak cation exchanger. The results of rate of sorption experiments reveal that the rate of sorption is high during the initial pe...

A method is described for the colorimetric determination of ammonia in plasma by a batch ion-exchange procedure. The method combines separation of ammonium ion, using a strongly acidic cation-exchange resin, with a sensitive colorimetric procedure which simultaneously elutes and colorimetrically reacts with the ammonium ion to produce a stable color. Interference by basic amino acids is negligi...

The objective of this study was to develop a simple and economical protocol for separating ovotransferrin from egg white. Egg white was separated from the yolk and diluted with the same volume of distilled water. To prevent denaturation during the separation process, ovotransferrin in 2x-diluted egg white was converted to its holo-form by adding 20 mM FeCl3.6H2O solution (0.25 to 3 mL/100 mL). ...

We developed an ion-exclusion/adsorption chromatography (IEAC) method employing a polystyrene-divinylbenzene-based weakly acidic cation-exchange resin (PS-WCX) column with propionic acid as the eluent for the simultaneous determination of multivalent aliphatic carboxylic acids and ethanol in food samples. The PS-WCX column well resolved mono-, di-, and trivalent carboxylic acids in the acidic e...

The use of cellulose-containing agricultural by-products modified with the cross-linking reagent dimethyloldihydroxyethylene urea (DMDHEU) and the quaternary amine, choline chloride, as anion exchange resins, has not been reported. The objective of the present study was to convert the readily available by-products, soybean hulls, sugarcane bagasse and corn stover to functional anion exchange re...

The methods employed previously in this laboratory for the isolation of oxytocin and vasopressin depend upon the use of several procedures to arrive at a highly purified sample. From the acetone powder of the posterior pituitary gland, the extraction and fractionation method of Kamm and coworkers (1) leads to a fraction rich in oxytocin and one rich in vasopressin, each at a stage of intermedia...

Proton-transfer reactions across and at the surface of biological membranes are central for maintaining the transmembrane proton electrochemical gradients involved in cellular energy conversion. In this study, fluorescence correlation spectroscopy was used to measure the local protonation and deprotonation rates of single pH-sensitive fluorophores conjugated to liposome membranes, and the depen...

Ion-exclusion chromatography (IEC) has been widely used in the separation of carboxylic acids, finding applications in the analysis of food and beverages, pharmaceuticals, biological samples, and acid rain samples.1 The retention mechanism of carboxylic acids in ion-exclusion chromatography has also been investigated in many studies, and the factors which affect retention can be divided into th...