This study evaluated the association between free fatty acid (FFA), ROS generation, mitochondrial dysfunction and bone mineral density (BMD) in type 2 diabetic patients and investigated the molecular mechanism. db/db and high fat (HF)-fed mice were treated by Etomoxir, an inhibitor of CPT1, MitoQ, and PFT-α, an inhibitor of P53. Bone metabolic factors were assessed and BMSCs were isolated and i...

Precipitation-use efficiency (PUE) is a key determinant of aboveground net primary production (ANPP). We used long-term datasets to contrast ANPP and PUE estimates between northern (southeast Montana) and southern (north Texas) mixed-grass prairies. Effects of varying amounts and temporal distribution of precipitation on PUE were examined at the Montana site, using a rainout shelter and irrigat...

OBJECTIVE The aim of this study was to describe the current use of pulmonary function tests (PFT) and the degree of matching between the diagnoses at referral and after the test. SUBJECTS AND METHODS A pre-test questionnaire was administered to 464 (5-88 years) patients referred to the PFT laboratory followed by a complete PFT test. The results of the patients' tests were analyzed in terms of...

Abstract Aim Though contraindicated in some respiratory laboratories due to a perceived increased risk of aneurysm rupture, pulmonary function tests (PFT) and cardiopulmonary exercise (CPET) are used commonly preoperative assessment patients with abdominal aortic (AAA) considered for elective repair. We examine whether PFT/CPET associated AAA rupture risk. Methods A retrospective analysis prosp...

PURPOSE Sarcoidosis affected lungs detected in more than 90% of patients. The relationship between different stages of pulmonary sarcoidosis and pulmonary function tests (PFT) as well as bronchoalveolar lavage fluid (BALF) cells can be established. Geographic and ethnic factors are known to be linked to the specific characteristics of patients with sarcoidosis. The purpose of the study was to e...

[1] Model-data fusion is a process in which field observations are used to constrain model parameters. How observations are used to constrain parameters has a direct impact on the carbon cycle dynamics simulated by ecosystem models. In this study, we present an evaluation of several options for the use of observations inmodeling regional carbon dynamics and explore the implications of those opt...

We have previously shown that protein prenylation occurs in the Trypanosomatids Trypanosoma brucei (T. brucei), Trypanosoma cruzi, and Leishmania mexicana and that protein farnesyltransferase (PFT) activity can be detected in cytosolic extracts of insect (procyclic) form T. brucei. A PFT that transfers the farnesyl group from farnesyl pyrophosphate to a cysteine that is 4 residues upstream of t...

The protein farnesyltransferase (PFT) 13-subunit gene of Saccharomyces cerevisiae, DPRI, was randomly mutagenized by PCR to construct a mutant DPRI gene library on a high-copy plasmid. The library was screened for suppression of the temperature sensitivity conferred by a mutation in the protein geranylgeranyltransferase type I (PGGT-I) 18-subunit gene, CALl. A mutant DPRI gene was identified wh...

Protein farnesylation is a form of posttranslational modification that occurs in most, if not all, eukaryotic cells. Inhibitors of protein farnesyltransferase (PFTIs) have been developed as anticancer chemotherapeutic agents. Using the knowledge gained from the development of PFTIs for the treatment of cancer, researchers are currently investigating the use of PFTIs for the treatment of eukaryo...