Several fixation and permeabilization techniques that enable the flow cytometric analysis of the cell contents have been introduced in recent years. These methods allow sensitive detection of intracellular antigens that facilitates the diagnosis of certain diseases. We have undertaken in this study to evaluate a simple method of fixation and permeabilization using 2% paraformaldehyde and Tween ...

PurposeCancer stem cells constitute an endless reserve for the maintenance and progression of tumors, they could be reason conventional therapy failure. New therapeutic strategies are necessary to specifically target them. In this context, microsecond pulsed electric fields have been selected expose D283Med cells, a human medulloblastoma cell line resulted rich in cancer normal astrocytes.Metho...

Paneth cells secrete alpha-defensins into the lumen from the base of small intestinal crypts, and cryptdin-4 (Crp4) is the most potent mouse alpha-defensin in vitro. Purified recombinant Crp4 and Crp4 variants with (des-Gly)-, (Gly1Val)-, (Gly1Asp)-, and (Gly1Arg)-substitutions were all bactericidal with Crp4 and (Gly1Arg)-Crp4 being slightly more active than other variants. Bactericidal activi...

Lysosomes serve as the cellular recycling centre and are filled with numerous hydrolases that can degrade most cellular macromolecules. Lysosomal membrane permeabilization and the consequent leakage of the lysosomal content into the cytosol leads to so-called "lysosomal cell death". This form of cell death is mainly carried out by the lysosomal cathepsin proteases and can have necrotic, apoptot...

the effects of pulsed electric field (pef) on the cell disintegration of sugar beet were studied. field strength (0.5kv/cm to 6kv/cm), pulse number (1 to 100 pulses) and capacity of capacitors (0.5 f to 32 f) were evaluated on degree of cell disintegration and consumed energy in pef treatment. the field conditions of 1 and 2 kv/cm, 8 f with 20 and 10 pulses respectively disintegrated the cel...