OBJECTIVE To further investigate peritoneal iron disruption in endometriosis by studying iron storage in peritoneal macrophages of patients with endometriosis compared with controls. DESIGN Cross-sectional study. SETTING Academic gynecology research unit in a university hospital. PATIENT(S) Fifty patients undergoing laparoscopy. INTERVENTION(S) Collection of peritoneal fluid samples (N ...

Peritoneal macrophages from germfree mice showed a higher basic activity of lysosomal enzymes than did macrophages from conventional mice, whereas oil-induced peritoneal influx, induction of lysosomal enzymes, and phagocytosis via the C3b receptor after endotoxin stimulation were reduced or absent. After germfree mice had been housed with conventional mice for 1 week, peritoneal influx and C3b ...

The subject of the study was the influence of two lines of transplantable melanomas on the secretion of cytokines (IL-6, TNF-alpha, OSM) and total proteins by hamster peritoneal macrophages. The results showed a statistically significant increase of total protein content and decrease of cytokine content in the supernatants of 24 h cultured macrophages from melanoma-bearing animals in comparison...

The ectoenzyme CD73 catalyzes the hydrolysis of AMP, and is one of the most important producers of extracellular adenosine. On regulatory T cells, CD73 is necessary for immunosuppressive functions, and on Th17 cells CD73-generated adenosine exerts anti-inflammatory effects. However, the expression and function of CD73 in pro-inflammatory M1 and in immunosuppressive M2 macrophages is largely unk...

Coxiella burnetii, the agent of Q fever in man and of coxiellosis in other species, is a small, dimorphic, obligate intracellular bacterium, sheltered within large, acidified, and hydrolase-rich phagosomes. Although several primary and established cell lines, macrophage-like cells, and primary macrophages from other species have been infected with C. burnetii, the infection of mouse primary mac...

The protein S100A9 plays a key role in the control of inflammatory response. The C-terminus of the murine S100A9 protein (mS100A9p) downregulates the spreading and phagocytic activity of adherent peritoneal cells. Murine peritoneal cells are constituted by macrophages and B-1 cells, and the latter exert an inhibitory effect on macrophage functions by secreting interleukin- (IL-) 10. Here, we in...

Certain activating immune receptors expressed on myeloid cells noncovalently associate with either DAP12 or FcepsilonRIgamma (FcRgamma chain), the ITAM-bearing transmembrane adapter proteins. An activating receptor, myeloid-associated Ig-like receptor (MAIR) II, is expressed on a subset of B cells and macrophages in the spleen and peritoneal cavity of mice and associates with DAP12 in these cel...

The phagocytic and microbicidal activities of alveolar and peritoneal macrophages were evaluated 4 and 24 h after a full-thickness scald burn of 26 to 28% body surface area in anesthetized rats. The contribution of serum factors to the macrophage functions was studied concurrently. The phagocytic activity of alveolar macrophages obtained 4 or 24 postburn was reduced approximately 65% below cont...

Trehalose-6,6'-dimycolate (cord factor; CF) injected into the peritoneal cavity of mice induced stimulation of the peritoneal macrophages, evidenced both by increased activity of the lysosomal enzyme, acid phosphatase, and by increased phagocytosis of Listeria monocytogenes. The increase in enzyme activity and phagocytosis was similar to that induced by killed or living BCG. When administered i...

The aim of this study was to evaluate macrophages repolarization from pro-inflammatory M1 to anti-inflammatory M2 phenotype upon transfection with microRNA-223 (miR-223) duplexes and miR-223 expressing plasmid DNA encapsulated in CD44-targeting hyaluronic acid-poly(ethyleneimine) (HA-PEI) nanoparticles (NPs). The HA-PEI/miR-223 NPs with spherical shape and an average diameter of 200 nm were eff...