The spontaneous increase in the transcription of the heat shock protein (hsp 70) gene in peripheral blood mononuclear cells of patients with active systemic lupus erythematosus (SLE) is shown by nuclear run on transcription assay. The transcription of hsp 70 gene in the peripheral blood mononuclear cells of five patients with active SLE was more than 10 times greater than that in five normal he...

RNA extracted from plasma and peripheral blood mononuclear cells of patients with chronic hepatitis C were used as the template for reverse transcription followed by double in vitro enzymatic amplification with nested primers. Hepatitis C virus was detected in 14 of 15 (93.3%) plasma specimens and in 8 of 15 (53.3%) peripheral blood mononuclear cell specimens obtained from patients with chronic...

Objective: Stevioside is a natural non-caloric sweetener which has been reported to have anti-inflammatory activity.  The aim of  the present study was to examine in vitro and in vivo effects of  stevioside on rats  plasma levels of tumor necrosis factor- α (TNF-α),  interleukin-1β (IL-1β), TNF-α and IL-1β release from lipopolysaccharide(LPS)-stimulated rat peripheral blood mononuclear cells (P...

Colonic tissue membrane binding to peripheral blood mononuclear leukocytes was quantitated by 1251labeling of membrane fragments and by determining the acquisition of membrane-specific enzyme activity and radioactivity in mononuclear cells after contact with the tissue membrane fragments. Mononuclear cells bound equal amounts of non mal and tumor tissue membrane fragments. Mononuclear cells cap...

Here we present retrieval of Peripheral Blood Mononuclear Cells by density-gradient medium based centrifugation for subsequent analysis of the leukocytes on an integrated microfluidic "Lab-on-a-Disc" cartridge. Isolation of white blood cells constitutes a critical sample preparation step for many bioassays. Centrifugo-pneumatic siphon valves are particularly suited for blood processing as they ...

OBJECTIVE To compare cytotoxic effects and antiviral efficacy of 9 nucleoside reverse transcriptase inhibitors (NRTIs) against FIV in feline peripheral blood mononuclear cells. SAMPLE Peripheral blood mononuclear cells obtained from 3 specific pathogen-free cats. PROCEDURES 3 of the 9 NRTIs had not been previously assessed in feline cell lines. Cytotoxic effects were determined by colorimet...

Objective(s): Recent progress in understanding the pathogenesis of premature ventricular contraction (PVC)-induced cardiomyopathy (PIC) has suggested a key role for inflammation. The aim of this study was to evaluate the expression of messenger RNAs (mRNAs) and the protein production of interleukin-6 (IL-6), IL-10, tumor necrosis factor alpha (TNF-α) and interferon-γ (...

BACKGROUND Abnormal interactions between red blood cells, leukocytes and endothelial cells play a critical role in the occurrence of the painful vaso-occlusive crises associated with sickle cell disease. We investigated the interaction between circulating leukocytes and red blood cells which could lead to aggregate formation, enhancing the incidence of vaso-occlusive crises. DESIGN AND METHOD...

background the rate of mother-to-infant hepatitis c virus (hcv) transmission is relatively low, however, this mode of infection is the most important route for childhood hcv involvement. the chances of transmission are increased by the level of maternal hcv viraemia and maternal human immunodeficiency virus (hiv) co infection. objectives the objective of the present study was to determine the p...

Abstract Background The application of DC-CIK in the field cancer immunotherapy has been shown to be an effective treatment. However, cost treatment is prohibitive for many patients, and lack standard manufacturing processes strategies are main limitations. Material Methods Our experiments used tumor lysate instead cell line as tumor-associated antigen source with DCs co-culture. We provide mos...