The production of kanamycin by three species of Streptonbyces and four fermentation media was studied. Streptomyces canus in medium No. 2 gave the highest yields of kanamycin aftrr 72 hrs. of fermentation. The presence of both molasses and glucose in the fermentation mcdium was an important factor for the production of kanamycin. The best concentration of molasses and glucose were 15.0 and 10.0...

Lactic acid bacteria, the object of current interest as bacteriocin producers, are microorganisms with complex requirements for peptidic sources, making them appropriate indicators for testing the suitability of formulations based on proteinaceous wastes for use as microbiological media. Different peptones obtained from visceral and fish muscle residues promoted growth of lactic acid bacteria w...

Bacterial growth and the production of ammonia from urea and by deamination of peptone has been examined at various pHs in both conventional static bacterial cultures and in a continuous cultivation system. Growth occurred on primary testing of 93 out of 100 strains of aerobic Gram-negative bacteria at pH 5, and 48 out of 50 strains of Esch. coli at pH 4.6. Hydrolysis of urea by Proteus mirabil...

Four strains of Corynebacterium diphtheriae were sent to UK participants in the UK National External Microbiological Quality Assessment Scheme, who were asked to examine the strains for toxin production by in vitro methods. Laboratories achieved 162/176 (92%) and 160/175 (91%) correct results with two rapid toxin producers and 145/175 (82%) with a slow toxin producer. With a non-toxigenic strai...

Effective control of outbreaks of Acanthaster planci represents the most immediate and practical intervention to reverse sustained declines in coral cover on reefs in the Indo-Pacific. This study explored the minimum doses of oxbile, oxgall, and thiosulfate-citrate-bile-sucrose agar (TCBS) that result in reliable and comprehensive mortality when injected into adult A. planci. The minimum doses ...

J. B. Egan and M. L. Morse (Biochim. Biophys. Acta 97:310, 1965) reported a defined medium which would support the growth of Staphylococcus aureus NCTC8511 for several generations if a peptone medium culture was diluted into it. In our hands, cultures of S. aureus NCTC8511 or S. aureus HS1159 (obtained from R. Z. Korman, Department of Physical Biology, Cornell University, Ithaca, N.Y.) maintain...

The present study conducted a genetic characterization and determined growth rate and biomass production in solid and liquid media, using strains obtained from wild edible sporomes of Lyophyllum that grow in high mountains. Vegetative isolation was used to obtain a total of four strains, which were divided into two clades within the section Difformia: Lyophyllum sp. and Lyophyllum aff. shimeji....