lsologous antigenic determinants of y-globulin revealed by proteolytic digestion of the globulin molecules were discovered by Osterland, Harboe, and Kunkel (1963) in human lgG and by Mandy, Fudenberg, and Lewis (1965) in rabbit y G. These determinants are detected by autologous and isologous antibodies found in most human sera and in all rabbit sera, respectively. These antibodies were named pe...

BACKGROUND Buckwheat is a common food in Japan, Korea and other countries. A candidate major buckwheat allergen, a 16-kDa protein (BWp16), was previously characterized as a pepsin-resistant protein associated with immediate-type allergies to buckwheat. However, whether recombinant BWp16 can react with a patient's IgE remains uncertain. METHODS The cDNA encoding BWp16 from Japanese buckwheat s...

1. Solubility curves of crude pepsin preparations indicate the presence of more than one protein. 2. One of these proteins has been isolated and crystallized and found to have constant activity and constant solubility in several solvents. 3. The solubility measurements are complicated by the unstable nature of the protein and the fact that in certain solvents the solubility of the protein is ma...

The experiments described above show that the rate of digestion and the conductivity of protein solutions are very closely parallel. If the isoelectric point of a protein is at a lower hydrogen ion concentration than that of another, the conductivity and also the rate of digestion of the first protein extends further to the alkaline side. The optimum hydrogen ion concentration for the rate of d...

Research on marine bioactive peptides has mainly focused on characterization of peptides in hydrolysates prepared withcommercial industrial enzymes and the usefulness of such hydrolysates in health and functional foods. However, a relevant questionis whether digestion of fish proteins with gastrointestinal proteases per se generates peptides that also can have health promotingproper...

Background: Alteration in polymorphism of tissue collagens has been reported in association with some genetic and metabolic disorders. These alterations can be estimated quantitatively by measuring alpha-chain monomers derived from the polymeric form of collagens following treatment with sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). We studied the rate of placental colla...