Molecular shape, flexibility, and surface hydrophilicity are thought to influence the ability of nanoparticles to cross biological barriers during drug delivery. In this study, coarse-grained (CG) molecular dynamics (MD) simulations were used to study these properties of a polymer-drug construct in potential clinical development: poly(γ-glutamyl-glutamate)-paclitaxel-poly(ethylene glycol) nonpe...

We report the synthesis and characterization of a series of multiblock thermoplastic polyurethane hydrogels synthesized with PEG as the soft segment and incorporating an isobutyl-functionalized POSS diol (TMP POSS diol) in the hard segment. The molecular weight of PEG was systematically varied to include 10, 20, and 35 kg/mol, while the molar ratio of POSS diol (as chain extender) to PEG was va...

This study aimed to develop an optimized depot injectable atorvastatin (ATR) biodegradable in situ gel (ISG) system with minimum initial burst using a central composite design. The factors selected were poly (d, l-lactide-co-glycolide) (PLGA) concentration (X1), molecular weight of polyethylene glycol (PEG) (X2), and PEG concentration (X3). The independent variables were the initial burst of AT...

Polyethylene glycol (PEG) is used in many applications, and the clinical field, it one of main components latest Covid vaccines. Its wide use justified by a relatively safe profile few known side effects. However, little about biophysical effects PEG on cells such as, cell stiffness, dry mass, total mass. Herein, exploiting digital holographic microscope, an inertial picobalance, nanoindenter, ...

In this study, micelles prepared from distearoylphosphatidylethanolamine with covalently attached poly(ethylene) glycol) (PEG) of molecular weight 2000 (DSPE-PEG-2000) were employed in micellar electrokinetic chromatography (MEKC) as pseudostationary phases. Since DSPE-PEG-2000 contains long hydrophobic alkyl chains, an anionic phosphate group, and hydrophilic PEG chains, the prepared micelles ...

The mechanism by which polyethylene glycol (PEG) mediates cell fusion has been studied by examining the movements of membrane lipids and proteins, as well as cytoplasmic markers, from erythrocytes to monolayers of cultured cells to which they have been fused. Fluorescence and freeze-fracture electron microscopy and fluorescence recovery after photobleaching have yielded the following results: (...

aqueous two-phase systems (atps) have  emerged as a powerful extraction method for the downstream processing of bio-molecules. the aim of this work was to investigate the possibility of utilizing atps for the separation of recombinant bacillus sphaericus phenylalanine dehydrogenase (phedh). polyethylene glycol (peg) and ammonium sulfate systems were selected for our experiment. the effect of di...

Large solutes such as high molecular weight proteins can be difficult to encapsulate in lipid vesicles. Passive trapping of these macromolecular solutes during vesicle formation typically results in concentrations inside the vesicles that are much lower than in the external solution. Here, we investigated the effect of macromolecular crowding on passive encapsulation of biological macromolecule...

This article reviews the use of multifunctional polymers founded on high-molecular weight poly(ethylene glycol) (PEG). The design of new PEG derivatives assembled in a dendrimer-like multimeric fashion or bearing different functionalities on the same molecule is described. Their use as new drug delivery systems based on the conjugation of multiple copies or diversely active drugs on the same bi...

Poly(ethylene glycol) (PEG) represents the gold standard for stealth polymers in polymer-based therapeutic delivery. Unfortunately, PEG has some limitations which warrant the examination of alternative polymers. High molecular weight PEG (>40 kDa) can accumulate in tissue, and in some patients, PEG can provoke an immunological response and/or an accelerated blood clearance upon repeated exposur...