In this study the forward and reverse primers were designated to amplify the segments (~250 bps and ~650 bps) of the gene coding domains 1 and 4 of aerolysin of Aeromonas hydrophila. These two domains are involved in pathogenesis of the aerolysin gene. Sequences for two restriction enzymes, Pst I and Hind III, were included in the forward and reverse primers respectively. These restriction enz...

purification of parasitic antigens is a major activity in immunoparasitology because of its application in immunodiagnosis, vaccination analysis of immunopathology, preparation of monocolomal antibody and finding out the cross reactive antigens versus specific antigens of a parasite. in this survey f2 ultrafiltration excretory secretory antigens of ascaris lumbricoides and toxocara cati were se...

the drb3 gene is a highly polymorphic major histocompatibility complex (mhc) class ii gene and plays an important role in variability of immune responsiveness and disease resistance. in the present study, the mhc class ii drb3 gene in water buffalo (bubalus bubalis) populations from northwest regions of iran was investigated through pcr-sscp. genomic dna was extracted from whole blood samples c...

A microwell hybridization assay was developed for the detection of the PCR products from both Mycobacterium tuberculosis complex bacteria and the recombinant Mycobacterium smegmatis strain 1008 that is used as an internal control to monitor inhibition in the PCR based on the M. tuberculosis complex-specific insertion sequence IS6110. The test is based on specific detection with digoxigenin-labe...

BACKGROUND Combination of CHD (chromo-helicase-DNA binding protein)-specific polymerase chain reaction (PCR) with electrophoresis (PCR/electrophoresis) is the most common avian molecular sexing technique but it is lab-intensive and gel-required. Gender determination often fails when the difference in length between the PCR products of CHD-Z and CHD-W genes is too short to be resolved. RESULTS...

S1 Fig. Verification of EBOV VLPs. (A) Protein purification of EBOVMS2 VLP. Proteins of various molecular weights were differentiated by gel exclusion chromatography. The target protein of EBOV VLP was obtained around 60mins. (B) The visual results of EBOV VLPs in SDS-polyacrylamide gel electrophoresis. The bands between 10KD and 15KD were EBOV VLPs. Lane M, prestained protein molecular weight ...

An integrated system with a nano-reactor for cycle-sequencing reaction coupled to on-line purification and capillary gel electrophoresis has been demonstrated. Fifty nanoliters of reagent solution, which includes dye-labeled terminators, polymerase, BSA and template, was aspirated and mixed with the template inside the nano-reactor followed by cycle-sequencing reaction. The reaction products we...

and Implications The ELISA assay is a more rapid and sensitive method for PCR product detection than conventional gel electrophoresis. The PCR/ELISA allows for the processing of numerous samples, is relatively inexpensive and eliminates the need for electrophoretic and photographic equipment as well as the use of potential carcinogens such as ethidium bromide which is utilized in the gel electr...

BACKGROUND Infestation of the skin by the "itch mite" Sarcoptes scabiei var. hominis results in a contagious skin infection in humans called "scabies". By resolving morphology issues, the present study was designed to be acquainted with itch mite by molecular markers. METHODS The mite samples were collected from scabies patients by visiting government hospitals of twin City, Pakistan. For suc...