A cryopreservation method and improved isolation techniques for detection of Pasteurella multocida from wetland samples were developed. Wetland water samples were collected in the field, diluted in dimethyl sulfoxide (DMSO, final concentration 10%), and frozen at -180 C in a liquid nitrogen vapor shipper. Frozen samples were transported to the laboratory where they were subsequently thawed and ...

pasteurella multocida (p. multocida), a gram-negative facultative anaerobic bacterium, is a causative animal pathogen in porcine atrophic rhinitis and avian fowl cholera. the outer membrane of gram-negative bacteria contains of many different protein in very high copy numbers. one of the major outer membrane, the h proteins have functional as high immunogenicity and antigenicity. in this study...

A total of 233 isolates of Pasteurella multocida were obtained from 2,912 cases of clinical respiratory disease in pigs in China, giving an isolation rate of 8.0%. Serogroup A P. multocida isolates were isolated from 92 cases (39.5%), and serogroup D isolates were isolated from 128 cases (54.9%); 12 isolates (5.2%) were untypeable. P. multocida was the fourth most frequent pathogenic bacterium ...

P. multocida is the causative agent of a wide range of diseases of animals, including fowl cholera in poultry and wild birds. Fowl cholera isolates of P. multocida generally express a capsular polysaccharide composed of hyaluronic acid. There have been reports of spontaneous capsule loss in P. multocida, but the mechanism by which this occurs has not been determined. In this study, we identifie...

Adhesion of bacteria to host cells is a crucial step in colonisation and disease pathogenesis. In this connection, further studies were conducted to analyse the attachment mechanisms of Pasteurella multocida to HeLa cells. Of 10 strains of P. multocida, belonging to serotypes A, B, D and E, all six of serotype A strains adhered strongly to HeLa cells. Exposure of the six strains to several phys...

To validate the identification of Pasteurella multocida-like bacteria negative for acid formation from sucrose, including isolates from bite wounds caused by large cats, 17 strains were phenotypically and genotypically characterized. Phylogenetic analysis of partially sequenced rpoB and infB genes showed the monophyly of the strains characterized and the reference strains of P. multocida. The s...

Pasteurella multocida is a Gram-negative bacterium that causes haemorrhagic septicaemia in cattle and buffaloes. These organisms are involved the formation of biofilm can evade treatment. There no definitive study to screen genes associated with production multocida. The present describes real-time PCR based approach for screening formation. Out 10 isolates screened formation, five them produce...

An effective vaccine for the immunization of sheep against Pasteurella multocida infection was prepared from P. multocida Strain D4 (Type D) and a selected strain of P. multocida Type A. Provided an adequate concentration of bacteria was used, the vaccine thus formulated induced antibodies in sheep that protected mice not only against the vaccine strains but also against infection by a number o...

To identify key regulators of subminimum inhibitory concentration (sub-MIC) antibiotic response in the Pasteurella multocida proteome, we applied systems approaches. Using 2D-LC-ESI-MS(2), we achieved 53% proteome coverage. To study the differential protein expression in response to sub-MIC antibiotics in the context of protein interaction networks, we inferred P. multocida Pm70 protein interac...

Potassium thiocyanate extracts of a virulent Pasteurella multocida 3:A rabbit isolate were prepared and used as a vaccine in rabbits. The extract contained protein, carbohydrate, hyaluronic acid, lipopolysaccharide, DNA, and RNA. The protein and lipopolysaccharide profiles of the extract were similar to those of the P. multocida cell membrane. Rabbits were vaccinated intranasally (i.n.) or intr...