Sequence heterogeneities in 16S rRNA genes from individual strains of Paenibacillus polymyxa were detected by sequence-dependent separation of PCR products by temperature gradient gel electrophoresis (TGGE). A fragment of the 16S rRNA genes, comprising variable regions V6 to V8, was used as a target sequence for amplifications. PCR products from P. polymyxa (type strain) emerged as a well-defin...

Biofertilizer can be used to replace chemical fertilizer so that soil quality is maintained and pollution avoided. The study aimed determine the effect of biofertilizers with different compositions microorganisms on growth maize plants. research was carried out in a greenhouse. Maize plants were fertilized by two kinds microorganisms. first biofertilizer contained Azotobacter sp, Azospirillum R...

Lignocellulosic biomass (LB) is a cost-effective, readily available, and attractive substrate to produce 2,3-butanediol (2,3-BD) commercially. Fermentative conversion of non-detoxified wheat straw hydrolysate (WSH) 2,3-BD was investigated using Paenibacillus polymyxa. The results show that P. polymyxa co-utilizes representative LB-derived sugars (glucose, xylose arabinose). Comparatively, ferme...

Paenibacillus polymyxa populations present in the rhizosphere of maize (cultivar BR-201) planted in Cerrado soil were investigated in order to assess their diversity at four stages of plant growth. A total of 67 strains were isolated and all strains were identified as P. polymyxa by classical biochemical tests, API 50CH tests and a set of species-specific primers based on the 23S rDNA sequence....

The neutral protease of Bacillus polymyxa had a broad pH optimum (6.0 to 7.2) for activity at 37 C. The enzyme was most stable at pH 5.6 to 5.8. The protease had an optimum temperature of 37 C and was quite thermostable up to 35 C, but at higher temperatures the stability decreased rapidly. The substrate specificity of the protease was similar to that of the neutral proteases of other members o...

To study nitrogen contribution to cucumber derived from fixation of Paenibacillus polymyxa WLY78. The nif gene cluster deletion mutant (?nifB-V) WLY78 was constructed by a homologous recombination method. effects plant-growth promotion were investigated greenhouse experiments. estimated 15N isotope dilution method (also being called the natural abundance technique). Deletion P. resulted in comp...

In this work, growth, cellular chemical composition and production of fusaricidin type antimicrobial compounds by P. polymyxa SQR-21 were compared in tryptone broth supplemented with four concentrations of iron (25, 50, 100 and 200 μM). The data revealed that the growth of P. polymyxa SQR-21 was increased by 3-8% with the increase in concentration of ferric ion (Fe). The production of fusaricid...