It has been postulated previously that a direct activation of all oxidative phosphorylation complexes in parallel with the activation of ATP usage and substrate dehydrogenation (the so-called each-step activation) is the main mechanism responsible for adjusting the rate of ATP production by mitochondria to the current energy demand during rest-to-work transition in intact skeletal muscle in viv...

The article provides information on the effect of pesticides - butylcaptax and droppa respiration oxidative phosphorylation liver mitochondria in rat rats their embryos. It has been shown that reduce oxidation succinate α-ketoglutarate V4*, V3 V dnf states drug conjugation pregnant animals most significant inhibition ADF formation respiratory chain fatal maternal occurs via NAD-dependent pathwa...

Oxidative phosphorylation in Escherichia coli membrane vesicles with a right-side-out orientation and loaded with ADP was investigated. Substrates of the electron transport chain could energize the phosphorylation of ADP, with the order of effectiveness being D-lactate greater than reduced phenazinemethosulfate greater than succinate greater than reduced nicotinamide adenine dinucleotide. Inhib...

Objective(s): To understand the role of mitochondrial respiration in cisplatin sensitivity, we have employed wild-type and mitochondrial DNA depleted Rho0 yeast cells. Materials and Methods: Wild type and Rho0 yeast cultured in fermentable and non-fermentable sugar containing media, were studied for their sensitivity against cisplatin by monitoring growth curves, oxygen consumption, pH changes ...

Many workers have produced evidence that in rat-liver mitochondria oxidative phosphorylation, adenosine triphosphatase and swelling are related activities (for reviews see Lehninger, 1962a; Racker, 1961). Oxidative phosphorylation of ratliver mitochondria is inhibited by low concentrations of trialkyltins (Aldridge & Cremer, 1955; Aldridge, 1958; Aldridge & Threlfall, 1961; Moore & Brody, 1961a...

This paper proposes a mechanism responsible for setting the sustainable level of muscle performance. Our contentions are that the sustainable work rate is determined (i) at the muscle level, (ii) by the ability to maintain ATP supply and (iii) by the products of glycolysis that may inhibit the signal for oxidative phosphorylation. We argue below that no single factor 'limits' sustainable perfor...

Work in the laboratories of Cohn (la-3) and Boyer (4, 5) has shown that intact mitochondria catalyze a rapid oxygen exchange reaction between inorganic phosphate and Hz0 of the medium, which can be followed by use of 018 as tracer. The dependence of the reaction on either oxidative phosphorylation or adenosine triphosphate and its sensitivity to 2,4-dinitropheno1 have indicated that the oxygen ...

It has been shown previously that direct stimulation of oxidative-phosphorylation complexes in parallel with the stimulation of ATP usage is able to explain the stability of intermediate metabolite (ATP/ADP, phosphocreatine/creatine, NADH/NAD+, protonmotive force) concentrations accompanied by a large increase in oxygen consumption and ATP turnover during transition from rest to intensive exerc...

In a complex enzymatic sequence the site of action of substrates and inhibitors is clearly marked by the way they affect the steady state concentrations of the components of the system (1). Antimycin A, for example, inhibits respiration in the succinic oxidase system (2) and at the same time increases the steady state reduction of cytochrome b and decreases that of cytochromes c, a, and aa (3)....

ATP synthase, also called F(1)F(o)-ATPase, catalyzes the synthesis of ATP during oxidative phosphorylation. The enzyme is reversible and is able to use ATP to drive a proton gradient for transport purposes. Our work has focused on the enzyme from Escherichia coli (ECF(1)F(o)). We have used a combination of methods to study this enzyme, including electron microscopy and chemical cross-linking. T...