pneumoniae ALI belonged to ST743, which is a newly identified sequence type. PCR mapping performed as described previously identified the blaOXA-48 gene that was located on the composite transposon Tn1999. Isolate ALI also possessed an intrinsic blaSHV-28 gene encoding a narrow-spectrum b-lactamase of the SHV-1 type. Plasmid location of the blaOXA-48 gene was confirmed by mating-out assays foll...

BACKGROUND In China, the spread and outbreak of OXA-48-producing Enterobacteriaceae remains largely unknown. METHODS OXA-48-producing isolates were analyzed for genetic relatedness by pulsed-field gel electrophoresis (PFGE), antimicrobial susceptibility by E-test, and sequence type (ST) by multilocus sequence typing. S1-PFGE and southern blotting were used for plasmid profiling, and PCR and s...

OBJECTIVES To assess the performance of three commercial molecular assays for detecting major families of carbapenemases in pure bacterial isolates. METHODS A panel of 450 isolates with previously defined carbapenem resistance mechanisms was tested using the Check-Direct CPE kit, the eazyplex(®) SuperBug complete A kit and the Xpert(®) Carba-R kit. Isolates included 438 Enterobacteriaceae and...

Introduction: Carbapenem resistance in Gram-negative bacilli (GNB) is a major concern the management of resistant infections. The mechanism carbapenem most commonly mediated by carbapenemases. five common genes (NDM, KPC, VIM, OXA, and IMP) are responsible for carbapenemase production. Knowledge these important disease. Objective: To estimate prevalence different production GNB at tertiary heal...

Laurent Dortet, Thierry Naas Associated French National Reference Center for Antibiotic Resistance, Le Kremlin-Bicêtre, France; Research Unit EA7361 “Structure, Dynamic, Function and Expression of Broad Spectrum β-Lactamases,” Faculty of Medicine, University Paris-Sud, Le Kremlin-Bicêtre, France; Department of Bacteriology-Parasitology-Hygiene, Bicêtre Hospital, Assistance Publique—Hôpitaux de ...