Purpose: To delve into the related molecular mechanism of suppressor cytokine signaling 4 (SOCS4) on cervical cancer cell proliferation and migration. Methods: Quantitative real-time polymerase chain reaction western blot assays were employed to examine SOCS4 mRNA or protein expression in four human line (HeLa, SW-732, AV3, CaSki) normal epithelium immortalized (H8) MTT viability applied verify...

Netrin-G1 (NTNG1) is a glycosyl-phosphatidylinositol-affixed synaptic adhesion molecule that participates in carcinoma developments. However, its underlying mechanism regulating prostate cancer (PCa) uncertain. NTNG1 mRNA and protein expressions tissue samples cells were demonstrated to be promoted using RT-qPCR western blot. Thereafter, CCK-8, overexpression accelerated PCa cell viability whil...

Skin is a unique target for vaccination. Targeting keratinocytes to produce proinflammatory mediators improve immunity rational approach vaccine design. Keratinocytes genetically engineered overexpress the stress response related transcription factor x-box binding protein 1 (XBP1) increased production of and co-delivered antigen. Keratinocyte-specific overexpression XBP1 through cutaneous genet...

Objective(s): Chemoresistance remains the main causes of treatment failure and mortality in cancer patients. There is an urgent need to investigate novel approaches to improve current therapeutic modalities and increase cancer patients' survival. Induction of drug efflux due to overexpression of                  P-glycoproteins is considered as an important leading cause of multidrug resistance...

Objective(s): To explore the correlation between expression patterns and functions of miR-383 and LDHA in hepatocellular cancer (HCC). Materials and Methods: We detected the expression of miR-383 and LDHA in 30 HCC tissues and their matched adjacent normal tissues using qRT-PCR. Then we performed MTT assay, foci formation assay, transwell migration assay, glucose uptake assay and lactate produc...

Background and objective: EGFR and HER-2 are two members of ERbB/HER family of Type I Transmembrane growth factor receptors. Cox2 is an enzyme responsible for the conversion of arachidonic acid to prostaglandins, which has a major role in angiogenesis and can modelate tumor growth. The aim of this study was to determine the level of expression of EGFR, HER-2 and Cox2 in colorectd cancer. Mater...

Background: Acinetobacter baumannii is commonly resistant to nearly all antibiotics due to presence of antibiotic resistance genes and biofilm formation. In this study we determined the presence of certain antibiotic-resistance genes associated with biofilm production and the influence of low iron concentration on expression of the biofilm-associated protein gene (bap) in development of biofilm...

Background: The majority of male patients with spinal cord injury (SCI) suffer from infertility. Nucleotide-binding oligomerization domain-like receptors NOD-like receptors (NLRs) are a kind of receptors that corporate in the inflammasome complex. Recent studies have introduced the inflammasome as the responsible agent for secreting cytokines in semen. Reactive oxygen species (ROS) is one of th...

Objective(s): The purpose of this study was to investigate the role of chloride channel protein 2 (ClC-2) in glutamate-induced apoptosis in the retinal ganglion cell line (RGC-5). Materials and Methods: RGC-5 cells were treated with 1 mM glutamate for 24 hr. The expression of ClC-2, Bax, and Bcl-2 was detected by western blot analysis. Cell survival and apoptosis were measured by 3-(4,5-dimeth...