Osteoclasts are bone-resorbing cells that are critical for the normal formation and maintenance of teeth and skeleton. Osteoclast deficiency can contribute to heterotopic ossification (HO), a pathology that is particularly detrimental to the mechanical functions of joints, valves and blood vessels. On the other hand, osteoclast over-activity is a major cause of osteoporosis. A reliable method f...

Psoriatic arthritis (PsA) is a chronic inflammatory joint disease with bone erosions mediated by osteoclasts. WNT signaling an important regulator of active osteoclastogenesis. We aimed to investigate how signal pathways regulate osteoclasticlastogenesis in PsA. The circulating CD14+ monocytes from 12 PsA patients (average age 45.2 years, M/F=8/4) and healthy controls (HCs) 47.4 M/F=7/5) were o...

Bisphosphonates are widely used anti-osteoporotic drugs targeting osteoclasts. They strongly inhibit bone resorption, but also reduce formation. This reduced formation is commonly ascribed to the mechanism maintaining resorption/formation balance during remodeling. The present study provides evidence for an additional where bisphosphonates actually impair onset of after resorption. based on mor...

Bone is a highly dynamic tissue characterized mainly by the interactions of osteoblasts and osteoclasts. When healing ability bone regeneration disturbed, targeted biophysical stimulations such as electrical stimulation are applied. In this study indirect effects electrically stimulated human on osteoclastogenesis were investigated to better understand detailed cellular interactions. Therefore,...

We demonstrated previously that a single injection of recombinant human macrophage colony-stimulating factor (rhM-CSF) is sufficient for osteoclast recruitment and survival in osteopetrotic (op/op) mice with a deficiency in osteoclasts resulting from a mutation in M-CSF gene. In this study, we show that a single injection of recombinant human vascular endothelial growth factor (rhVEGF) can simi...

BACKGROUND Osteoclasts secrete tartrate-resistant acid phosphatase (TRAP; EC 3.1.3.2) 5b into the circulation. We studied the release of TRAP 5b from osteoclasts using a mouse in vitro osteoclast differentiation assay. METHODS We developed and characterized a polyclonal antiserum in rabbits, using purified human osteoclastic TRAP 5b as antigen. The antiserum was specific for TRAP in Western a...

Osteoclasts are multinucleate giant cells playing key roles in bone resorption. These cells solubilize mineralized bone matrix by means of acid and protease action; however, the precise mechanism of this process is not well known. Recently, we succeeded in the isolation of pure osteoclasts from rabbit bones and constructed a cDNA library. Using a differential screening procedure, two genes expr...

We incidentally found that osteoclast precursors and mature osteoclasts express Fas ligand (FasL) as well as Fas, which was confirmed by flow cytometry, immunofluorescent staining, and RT-PCR. The aim of this study was to determine the role of FasL in differentiation and cell death of osteoclasts. To study the role of FasL in osteoclastogenesis, neutralizing anti-FasL mAb or rFasL was added dur...