OBJECTIVE To determine whether the activation of metalloproteinases can be achieved by the interaction of the inflammatory cytokine, IL-1 beta, with rheumatoid osteoblasts. RESULTS MMP-2 is not secreted by rheumatoid osteoblasts as a proenzyme; however, IL-1 beta stimulation induced the secretion of MMP-2 as an active form from rheumatoid osteoblasts. This MMP-2 activating activity was detect...

OBJECTIVE Extra corporeal shock waves (ESW) have been proposed as additional therapy in bone fracture repair and osteoarthrtitis (OA). However, little is known on the effects of ESW on osteoblast metabolism. The aim of this study was to evaluate phenotype changes of healthy and OA human osteoblasts following ESW treatment. METHODS Osteoblasts were isolated from subchondral bone of 13 OA patie...

Osteoclasts develop from monocyte-macrophage lineage cells under the regulation of osteoblasts. Osteoblasts express two cytokines essential for osteoclastogenesis, macrophage colony-stimulating factor (M-CSF) and receptor activator of nuclear factor-KappaB ligand (RANKL). Osteoblasts also produce osteoprotegerin (OPG), a decoy receptor for RANKL, which inhibits the interaction between RANKL and...

N-methyl-D-aspartate (NMDA) receptors for the central neurotransmitter l-glutamate (Glu) have been shown to be present in both osteoblasts and osteoclasts. Sustained exposure to the NMDA channel antagonist dizocilpine (MK-801) significantly prevented increases in both alkaline phosphatase activity and Ca2+ accumulation in a concentration-dependent manner in osteoblasts cultured for 7-28 days in...

In an attempt to increase the life of cementless prostheses, an hydroxyapatite-coated implant which releases a bisphosphonate has been suggested as a drug-delivery system. Our in vitro study was designed to determine the maximum dose to which osteoblasts could be safely exposed. Our findings demonstrated that zoledronate did not impair the proliferation of human osteoblasts when used at concent...

Bone is a dynamic organ that, once formed, undergoes constant remodeling process that includes bone resorption and synthesis. Osteoclasts osteoblasts are primarily responsible for controlling this process. X-box binding protein 1 (XBP1), transcription factor, affects the metabolism of bones in various ways. In recent years, numerous studies have revealed XBP1 plays vital role metabolism, includ...

NOTUM produced by osteoblasts is known to regulate cortical bone mass. Our new findings show that specifically derived DMP1-expressing osteocytes and late regulates mass not trabecular

conclusions based on our findings, we conclude that the separation and reproduction of adipose tissue cells is an appropriate method for purification of mscs in animal studies. regarding the histomorphometric and flow cytometry analysis results, we demonstrated the differentiation ability of mscs in normal medium and hope to employ these cells for the regeneration of damaged bone tissues in the...

This integrative review aimed to report the toxic effect of submicron and nano-scale commercially pure titanium (cp Ti) debris on cells peri-implant tissues. A systematic search was carried out PubMed electronic platform using following key terms: Ti “OR” “AND” dental implants nanoparticles nanometric osteoblasts “OR “cytotoxicity” OR “macrophage” “mutagenic” “peri-implantitis”. The inclusion c...

Craniosynostosis (CS), the premature ossification of cranial sutures, is attributed to increased osteogenic potential of resident osteoblasts, yet the contribution of the surrounding extracellular matrix (ECM) on osteogenic differentiation is unclear. The osteoblast-secreted ECM provides binding sites for cellular adhesion and regulates the transport and signaling of osteoinductive factors secr...