Web Services orchestrations require a firm mathematical basis for their development. We start from the Orc formalism proposed by J. Misra and co-workers, at Austin University. Orc is small and elegant and captures the essence of orchestrations. We translate Orc into colored Petri net systems, a generalization of Petri nets allowing to handle recursion—this formalism was recently proposed by Dev...

Turbo-expander is the most crucial and expensive component in Organic Rankine Cycle (ORC) power generation systems. The turbine engineering, design and research development process for ORC system is costly, time-consuming, and difficult for new-entrants in the ORC field. This paper investigates the re-design and retrofit of an off-the-shelf turbocharger for use in a 1 kW ORC system. The expande...

Open radical cystectomy (ORC) is the current gold standard treatment for muscle invasive bladder cancer. As surgeons become more proficient in minimally invasive and robotic surgical techniques, the number of patients undergoing robotic-assisted radical cystectomy (RARC) is increasing. Although minimally invasive methods are on the rise, research that critically compares open surgery with robot...

The origin recognition complex (ORC) in yeast is a complex of six tightly associated subunits essential for the initiation of DNA replication. Human ORC subunits are nuclear in proliferating cells and in proliferative tissues like the testis, consistent with a role of human ORC in DNA replication. Orc2, Orc3, and Orc5 also are detected in non-proliferating cells like cardiac myocytes, adrenal c...

PURPOSE Robot-assisted radical cystectomy (RARC) was originally intended to replace open radical cystectomy (ORC) as a minimally invasive surgery for patients with invasive bladder cancer. The purpose of this study was to evaluate the advantages of robotic surgery, comparing perioperative and oncologic outcomes between RARC and ORC. MATERIALS AND METHODS Between June 2012 and August 2016, 49 ...

In budding yeast, the eukaryotic initiator protein ORC (origin recognition complex) binds to a bipartite sequence consisting of an 11 bp ACS element and an adjacent B1 element. However, the genome contains many more matches to this consensus than actually bind ORC or function as origins in vivo. Although ORC-dependent loading of the replicative MCM helicase at origins is enhanced by a distal B2...

In this study, two systems are brought forward to recover the waste heat of a proton exchange membrane fuel cell (PEMFC), which are named the organic Rankine cycle (ORC), and heat pump (HP) combined organic Rankine cycle (HPORC). The performances of both systems are simulated on the platform of MATLAB with R123, R245fa, R134a, water, and ethanol being selected as the working fluid, respectively...

The origin recognition complex (ORC) is an essential component of the prereplication complex (pre-RC) in mitotic cell cycles. The role of ORC as a foundation to assemble the pre-RC is conserved from yeast to human. Furthermore, in metazoans ORC plays a key role in determining the timing of replication initiation and origin usage. In this report we have produced and analyzed a Drosophila orc1 al...

Purification of the origin recognition complex (ORC) from wild-type budding yeast cells more than two decades ago opened up doors to analyze the initiation of eukaryotic chromosomal DNA replication biochemically. Although revised methods to purify ORC from overproducing cells were reported later, purification of mutant proteins using these systems still depends on time-consuming processes inclu...

Eukaryotic origins of DNA replication are bound by the origin recognition complex (ORC), which scaffolds assembly of a pre-replicative complex (pre-RC) that is then activated to initiate replication. Both pre-RC assembly and activation are strongly influenced by developmental changes to the epigenome, but molecular mechanisms remain incompletely defined. We have been examining the activation of...