The present work is aimed at assessing the impact of strong column-weak beam (SCWB) criterion on seismic performance of optimally designed steel moment frames. To this end, different SCWB ratios are considered for steel special moment resisting frame (SMRF) structures and performance-based design optimization process is implemented with the aid of an efficient metaheuristic. The seismic collaps...

The strategies employed in chromatography steps play a key role in downstream processes for monoclonal antibody (mAb) manufacture. This work addresses the integrated optimisation of chromatography step sequencing and column sizing in mAb purification processes. Chromatography sequencing decisions include the resin selection at each typical step, while the column sizing decisions include the num...

The commercial availability of random peptide libraries displayed on the M13 phage is increasing their use forstudies on epitope identification, enzyme inhibitors, receptor ligands, etc. In this study two experimentswhere planned for selection of peptides. First with sheep antibodies, the positive selector was IgG, preparedon Protein G column from a pool of 11 sheeps immunized...

Brucellosis is caused by the bacterium Brucella and affects various domestic and wild species. GroEL (Heat Shock Protein 60kDa) as one of the major antigens that stimulate the immune system, increases Brucella survival. The aim of the current study was to clone and express GroEL in Escherichia coli in order to design subunit vaccine. Amplifying was performed using specific primers. The full-len...

This article deals with the issues associated with developing a new design methodology for the nonlinear model-predictive control (MPC) of a chemical plant. A combination of multiple neural networks is selected and used to model a nonlinear multi-input multi-output (MIMO) process with time delays.  An optimization procedure for a neural MPC algorithm based on this model is then developed. T...

introduction: gene expression and purification of luciferases from the firefly, lampyris turkestanicus, and optimization of cellular atp measurements were performed. methods: cdna encoding luciferases from lampyris turkestanicus was transferred from pqe30 vector into pet28a expression vector and pltu28 was built. newly constructed vector was expressed in e. coli xl1 blue and the recombinant luc...