objective: the present study aimed to simultaneously evaluate the association between expression of three potential factors [post-acrosomal sheath ww domain-binding protein (pawp), phospholipase cζ (plcζ), and truncated form of the kit receptor (tr-kit)] as candidates of oocyte activation with fertilization rate and early embryonic development. materials and methods: in this experimental study,...

In recent years, the uptake of assisted reproductive techniques such as in vitro fertilisation has risen exponentially. However, there is much that is still not fully understood about the biochemical modifications that take place during the development and maturation of the oocyte. As such, it is essential to further the understanding of how oocyte manipulation during these procedures ultimatel...

The ovarian follicle encloses oocytes in a microenvironment throughout their growth and acquisition of competence. Evidence suggests a dynamic interplay among follicular cells and oocytes, since they are constantly exchanging "messages". We dissected bovine ovarian follicles and recovered follicular cells (FCs-granulosa and cumulus cells) and cumulus-oocyte complexes (COCs) to investigate wheth...

OBJECTIVE To compare the outcomes of embryo development between oval-shaped oocyte as an abnormal morphology oocyte and a normal morphology oocyte in In Vitro Fertilization (IVF). METHODS This study was a comparative analytical study with retrospective approach which lasted from September 2014 until November 2015. For this study, we used secondary data (medical records) from 24 patients submi...

INTRODUCTION In women showing impaired fertility, a decreased response to ovarian stimulation is a major problem, limiting the number of oocytes to be used for assisted reproduction techniques (ART). Despite the several definitions of poor response, it is still a matter of debate whether young poor responder patients also show a decrease in oocyte quality. The objective in this study was to inv...

A decline in female reproduction is one of the earliest hallmarks of aging in many animals, including invertebrates and mammals [1-4]. The insulin/insulin-like growth factor-1 signaling (IIS) pathway has a conserved role in regulating longevity [5] and also controls reproductive aging [2, 6]. Although IIS transcriptional targets that regulate somatic aging have been characterized [7, 8], it was...