نتایج جستجو برای: nucleic acid sequencing
تعداد نتایج: 867536 فیلتر نتایج به سال:
Nucleic acid therapeutics are limited by inefficient delivery to target tissues and cells and by an incomplete understanding of how nanoparticle structure affects biodistribution to off-target organs. Although thousands of nanoparticle formulations have been designed to deliver nucleic acids, most nanoparticles have been tested in cell culture contexts that do not recapitulate systemic in vivo ...
5.1 DNA Sequence Determination 168 5.1.1 Principles of DNA Sequencing 168 5.1.2 Automated Fluorescent DNA Sequencing 169 5.1.3 RNA Sequencing by Reverse Transcription 170 5.2 Gene Cloning 170 5.2.1 Classical Cloning 170 5.2.2 The Polymerase Chain Reaction 173 5.3 Enzymes Useful in Gene Manipulation 174 5.3.1 Restriction Endonucleases 174 5.3.2 Other Nucleases 175 5.3.3 Polynucleotide Kinase 176...
High-throughput sequencing is subject to sequence dependent bias, which must be accounted for if researchers are to make precise measurements and draw accurate conclusions from their data. A widely studied source of bias in sequencing is the GC content bias, in which levels of GC content in a genomic region effect the number of reads produced during sequencing. Although some research has been p...
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