We have produced a murine monoclonal antibody (MAb), ZX10, recognizing the NTPase/helicase domain of the hepatitis C virus (HCV) nonstructural 3 protein (NS3), from which we designed a single-chain variable fragment (ScFv). The ZX10 MAb recognized a discontinuous epitope of the NTPase/helicase domain, of which the linear sequence GEIPFYGKAIPL at residues 1371 to 1382 constitutes one part. cDNAs...

The hepatitis C virus (HCV) nonstructural 3 protein (NS3) is a 70-kDa multifunctional enzyme with three known catalytic activities segregated in two somewhat independent domains. The essential machinery of a serine protease is localized in the N-terminal one-third of the protein, and nucleoside triphosphatase (NTPase) and helicase activities reside in the remaining C-terminal region. NS4A is a ...

Background: An expanded range of contraceptive methods could reduce unintended pregnancies. User preferences research is important for successful development products people want to us. This paper describes four approaches soliciting user input in different ways: 1) perspectives on method characteristics, 2) reactions development, 3) trade-offs between and 4) “blue-s...

Background: The elicitation of inconsistent health-state utility values (HSUVs) is a prevalent problem. There are two approaches to address this problem: (1) intervention during the process ensure that patients estimate consistent HSUVs; (2) no and HSUVs adjusted after fact. This paper studies three models recently proposed for adjusting some may consider unrealistic...

Background: The primary objective worldwide during the coronavirus disease 2019 (COVID-19) pandemic has been controlling transmission. However, lockdown measures used to mitigate transmission have affected human behavior and altered lifestyles, with a likely impact on chronic non-communicable diseases. More than year into pandemic, substantia...

Background: The effect of solid retention time (SRT) over cheese whey substrates in a fermentation process drives changes the composition polyhydroxyalkanoates (PHAs) obtained. Volatile fatty acids produced first step an anaerobic sequencing batch reactor were used as to produce PHA using mixed microbial cultures under aerobic dynamic feeding con...

Background: The SARS-CoV-2 pandemic is a global threat to humans and the world’s economy. Effective safe vaccines against this virus are essential control eradicate pandemic. currently applied carry spike-protein mRNA/cDNA. These go through several cellular processes in recipients for producing antigens. On contrary, RBD (receptor binding domain)-protein an antigen. ...

The World Health Organization recently launched its 2021-2030 roadmap, Ending the Neglect to Attain Sustainable Development Goals, an updated call arms end suffering caused by neglected trop...

Background: Nurses who are in charge of the Neonatal Intensive Care Units (NICUs) have a different workload and work assignments compared to other units. Evidence suggests that higher nurse workloads will increase risk missed nursing care. Missed care NICU eventually worsen neonatal prognosis. This is major problem developing countries, which currently still high mor...

Background Integrated metrics that account for resource use and human health are essential to help identify support development pathways safeguard planetary health. We countries achieved the highest levels of at lowest cost Earth’s natural capital report ecological within-country analyses associations between indicators environmental sustain...