Nucleated red blood cells (NRBCs) are premature erythrocyte precursors that reside in the bone marrow of humans all ages as an element erythropoiesis. They rarely present healthy adults’ circulatory systems but can be found circulating fetuses and neonates. An NRBC count is a cost-effective laboratory test currently used everyday clinical practice; it mostly diagnosis hematological diseases/dis...

Data regarding the quantification of CD34+ hematopoietic stem cell HSC in dual-platform (DP) approach using a hematological analyzer featuring dedicated nRBC channel are limited if not inexistent. The objective this study is to compare performance DP on new generation channel, Sysmex XN-1000, with single-plate (SP) form Beckman Coulter’s Navios flow cytometer. Using International Society for He...

In a total of 42 consecutive pregnancies with intrauterine growth retardation (IUGR), Doppler velocimetry was related to neonatal outcome as determined by Apgar score at 1 min, umbilical artery acid-base status and PO2, number of nucleated red blood cells (NRBC), duration of ventilatory support, and sonographic appearance of cerebral leukomalacia. Doppler flow velocity waveforms were obtained f...

Fluorescence flow cytometry enhances analytical possibilities beyond the normal 5-part differential by providing excellent cluster resolution and separation of abnormal blood cells. A nucleated red blood cell count (NRBC) and an immature granulocyte count (IG) are part of an extended differential on the xe systems with the potential to improve the diagnostic usefulness of the routine 5-part dif...

Background: Sepsis is defined as a systematic inflammatory reaction against infection. Despite the recent advances in medical and pediatric sciences, sepsis remains a significant life-threatening health issue. The main problem associated with this disorder is the nonspecific and non-discriminative symptoms of noninfectious diseases. The present study aimed to introduce diagnostic parameters of ...

which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. A 44-year-old woman presented with myalgia and fever. Initial laboratory results were: White blood cell (WBC) count, 7.8×10 9 /L; hemoglobin, 11.3 g/dL; platelet count, 42×10 9 /L; and lactate dehydrogenase, 619 IU/L. Peripheral blood cell morphology showed ...

BACKGROUND AND PURPOSE Neonatal hypoxic-ischemic encephalopathy (HIE) is one of the leading causes of neurological handicap in developing countries. Human umbilical cord blood (hUCB) CD34-positive (CD34⁺) stem cells exhibit the potential for neural repair. We tested the hypothesis that hUCB CD34⁺ stem cells and other cell types [leukocytes and nucleated red blood cells (NRBCs)] that are up-regu...

The bone marrow aspiration test conventionally has been performed by visual methods, using a light microscope, because automatic blood cell analyzers cannot adequately capture erythroblasts and immature granulocytes (IGs) (Tatsumi et al.: Osaka City Med J 1988;34:135-146; Tatsumi et al.: Am J Clin Pathol 1986;86:50-54). With the development of the XE-2100 automatic blood cell analyzer (Sysmex C...

We evaluated the new Beckman Coulter DxH 800 hematology analyzer (Beckman Coulter, Miami, FL) vs the Abbott Diagnostics Cell-Dyn Sapphire (Abbott Diagnostics, Santa Clara, CA) and Beckman Coulter LH 780 hematology analyzers using 430 adult specimens. The DxH 800 provided a CBC and differential that correlated well with those of the Sapphire and LH 780, with most parameters showing correlation c...

The detection and genetic analysis of fetal cells in maternal blood will permit noninvasive prenatal screening for genetic defects. Applied Imaging has developed and is currently evaluating a system for semiautomatic detection of fetal nucleated red blood cells on slides and acquisition of their DNA probe FISH images. The specimens are blood smears from pregnant women (9-16 weeks gestation) enr...