In this study, a new formulation of low-cost, biodegradable, and non-toxic biosurfactant by Candida sphaerica UCP 0995 was investigated. The study conducted in bioreactor on an industrial waste-based medium, central composite rotatable design used for optimization. best results, namely 25.22 mN/m reduction surface tension, yield 10.0 g/L, critical micelle concentration 0.2 were achieved 132 h a...