The four components of the genome of cowpea chlorotic mottle virus have been prepared in a highly active and highly purified state by a method based on their resolution by polyacrylamide gel electrophoresis. Activity and purity have been confirmed by gel electrophoresis under denaturing and non-denaturing conditions, infectivity tests on whole plants, translation in an mRNA dependent rabbit ret...

We have developed an electrophoretic method to determine the size of a protein—DNA complex using the same binding and electrophoresis conditions as in an electrophoretic mobility shift assay (EMSA). The method is an adaptation of that of Ferguson (1), originally developed to determine the size of native proteins in non-denaturing gel systems. In SDS polyacrylamide gel electrophoresis, associati...

Solubilization of sewage sludge creates favorable conditions for anaerobic microorganisms to produce biogas. In this paper, we quantify the effect of heating pretreatment on the degree of solubilization of sewage sludge. The pretreatment process was carried out using a lab-scale industrial microwave unit (2450 MHz frequency). Response surface analysis was applied to determine the combination of...

the purposes of this research were to the evaluation of resistance to nickel and cadmium in 52 indigenous and non-indigenous strains of contaminated soils and a study of growth promoting characteristics of some resistant isolates. resistance test at three levels of cadmium (0, 100, 200 mg l-1) and five levels of nickel (0, 250, 500, 750, 1000 mg l-1) in the framework of a completely randomized ...

calpastatin (cast) is a specific inhibiter of calpains, playing a role in meat tenderization and myogenesis. in the present study, the polymorphism of the cast gene of makoei sheep was investigated by polymerase chain reaction and single strand conformation polymorphism technique (pcr–sscp). genomic dna was extracted from whole blood samples collected from 100 sheep. a 622 bp cast exon 1 segmen...

To expand the applicability of the biotin-(strept)avidin system, a biotin analog with reversible binding under non-denaturing conditions has been designed, and its applications in protein labeling, detection, and isolation have been evaluated.

The effect of Triton X-100, Na cholate and Tween 80 on the solubilization of integral membrane proteins in intact cells, spheroplasts and inner-membrane fragments of Salmonella typhimurium was studied. The detergents were used in various concentrations (1.6 to 64 mM) and cytochromes b and d were used as marker to monitor the solubilization of membrane-bound proteins. Results showed that no inne...

Urea is an effective solubilizer for dyes with low aqueous solubility. To establish, at a molecular level, the reason behind action of urea as solubilizer, we employ rigorous statistical thermodynamics approach based on Kirkwood-Buff theory solutions. We show that (i) contrary to classical hypothesis “water structure breaking”, effect dye hydration makes minor contribution solubilization; (ii) ...

The solubilization dynamics of dimyristoylphosphatidylcholine (DMPC) liposomes, as induced by sodium dodecyl sulfate (SDS), were investigated; this investigation was motivated by several types of atypical behavior that were observed in the solubilization in this system. The liposomes and surfactants were mixed in a microchip, and the solubilization reaction of each liposome was observed using ...