background: the assessment of altered nitric oxide (no) availability is of potentially important diagnostic and prognostic significance. the present study is aimed to investigate the effect of l-arginine (as a natural no donor) supplementation on no metabolite in a rabbit model of hypercholesterolemia to find a reliable marker for endothelial no production. methods: white male rabbits (n = 30) ...

Objective To observe the analgesic effect of early hyperbaric oxygen (HBO) treatment in chronic constriction injury (CCI) rats, and to analyze the influence of HBO on the expression of neuronal nitric oxide synthase and inducible nitric oxide synthase and on the levels of inflammatory factors. Methods Rats were assigned into three groups randomly: sham, CCI, and HBO groups. The CCI rat model wa...

PURPOSE The cascade of molecular events leading to tumor necrosis factor (TNF)-mediated tumor regression is still incompletely elucidated. We investigated the role of endothelial nitric oxide synthase in determining the tumor-selective activity of TNF. EXPERIMENTAL DESIGN Using quantitative real-time PCR, endothelial nitric oxide synthase gene levels were measured in melanoma metastases of th...

Background: The assessment of altered nitric oxide (NO) availability is of potentially important diagnostic and prognostic significance. The present study is aimed to investigate the effect of L-arginine (as a natural NO donor) supplementation on NO metabolite in a rabbit model of hypercholesterolemia to find a reliable marker for endothelial NO production. Methods: White male rabbits (n = 30)...

Endothelial production of nitric oxide (NO) is critical for vascular homeostasis. Nitrite and nitrate are formed endogenously by the stepwise oxidation of NO and have, for years, been regarded as inactive degradation products. As a result, both anions are routinely used as surrogate markers of NO production, with nitrite as a more sensitive marker. However, both nitrite and nitrate are derived ...

BACKGROUND Inducible nitric oxide synthase (iNOS) is expressed and is functionally active in the presence of transplant arteriosclerosis. However, the early involvement of iNOS in alterations of microvascular endothelial function in the absence of preexisting lesions remains unclear; this information would be of prognostic value. We studied the course of iNOS mRNA expression, transcardiac nitri...

Introduction: Ischemia/reperfusion (IR) injury involves a complex interrelated sequence of events. High levels of nitric oxide (NO) are generated with inducible form of nitric oxide synthase (iNOS) leading to the renal IR injury and glutathione (GSH) depletion. The present study was designed to investigate the effect of L-Nil (N6- (1-Iminoethyl)-L- lysine.hydrochloride), a selective inhibito...

BACKGROUND Previous work has suggested that in the liver, adenosine preconditioning is mediated by nitric oxide. Whether the endothelial isoform of nitric oxide synthase plays a part in this mechanism has however not yet been investigated. METHODS Wistar rats were used (6 in each group)--Groups: (1) sham, (2) ischemia-reperfusion, (3) adenosine + ischemia-reperfusion, (4) endothelial isoform ...

Vasoplegic shock refractory to vasopressors is associated with significant morbidity and mortality. Catecholamine driven therapies are first-line in the management of vasoplegic shock. Research regarding use non-catecholamine agents setting lacking. Methylene blue has multiple off-labelled indications for use, but recently been suggested have a role treating mediated by dysregulation nitric oxi...

–Stimulation of basal forebrain neurons elicits regional cerebral blood flow increases which are reportedly mediated by acetylcholine and nitric oxide. However, the modality of interaction between these two mediators remains unclear. Particularly, little is known about the source, i.e. endothelial, glial and/or neuronal, of the potent gaseous vasodilator nitric oxide. In the present study, we e...