flaviviridae are viruses that cause several diseases including dengue fever, japanese encephalitis, murray valley encephalitis, tick-borne encephalitis, west nile encephalitis, yellow fever and hepatitis c virus infection. members of this family have monopartite, linear, single-stranded rna genomes of positive polarity, 9.6-12.3 kb in length. here, we have analyzed the codon usage of 13 species...

West Nile encephalitis was first identified in 1937, but until now, it was never diagnosed in Korea. A 58-yr-old Korean man was admitted with headache and cognitive dysfunction. The patient had been on a business trip in Guinea. Cerebrospinal fluid (CSF) showed pleocytosis. The patient complained of both leg weakness,and arachnoiditis and myelitis were observed on lumbar magnetic resonance imag...

Determining how West Nile virus crosses the blood-brain barrier is critical to understanding the pathogenesis of encephalitis. Here, we show that ICAM-1(-/-) mice are more resistant than control animals to lethal West Nile encephalitis. ICAM-1(-/-) mice have a lower viral load, reduced leukocyte infiltration, and diminished neuronal damage in the brain compared to control animals. This is assoc...

West Nile virus infection in humans is mostly asymptomatic. Less than 1% of neuro-invasive cases show a fatality rate of around 10%. Acute flaccid paralysis of respiratory muscles leading to respiratory failure is the most common cause of death. Although the peripheral nervous system can be involved, isolated phrenic nerve palsy leading to respiratory failure is rare and described in only two c...

contemporary poets and writers use from their past legacy and their work influenced by the relationship to the bad or good works of the past writers whom they revealed strength and weakness of the past societies. najib mahfouz used past legacy in his work by “thartharafawq al-nil (“chatter on the nile”) and animate and quicken his novel with this skill. he used kinds of past legacies such as hi...

We report the generation of West Nile virus (WNV) infectious clones for the pathogenic lineage 1 Texas-HC2002 and nonpathogenic lineage 2 Madagascar-AnMg798 strains. The infectious clones exhibited biological properties similar to those of the parental virus isolates. We generated chimeric viruses and found that viral factors within the structural and nonstructural regions of WNV-TX contribute ...

West Nile virus is a mosquito-borne flavivirus and human neuropathogen. Since the virus was recognized in New York City in 1999, it has spread rapidly across the United States, with human disease documented in 39 states and the District of Columbia. West Nile virus can cause a broad range of clinical syndromes, including fever, meningitis, encephalitis, and a flaccid paralysis characteristic of...

Neutral lipid droplets (LDs) are dynamic lipid storage organelles found in all eukaryotic cells from yeast to mammals and higher plants. LDs are important to many physiological processes that include basic cellular maintenance, metabolism, and diverse medical pathologies. LD accumulation has been studied extensively by a range of methods, but particularly by microscopy with several fluorescent ...

Flaviviruses are positive-stranded RNA viruses that are a public health problem because of their widespread distribution and their ability to cause a variety of diseases in humans. West Nile virus is a mosquito-borne member of this genus and is the etiologic agent of West Nile encephalitis. Clinical manifestations of West Nile virus infection are diverse, and their pathogenic mechanisms depend ...

Since its emergence in the United States in 1999, West Nile Virus has caused hundreds of deaths and has been spreading geographically. In order to efficiently control West Nile Virus outbreaks, it is necessary to monitor the spread of its primary mosquito vectors. This report contains a new computational method to optimize the surveillance methods of West Nile Virus vectors at St. Tammany Paris...