conclusions: vegetables are a new source of h. pylori and accurate washing of vegetables improves such contaminations. results: the results showed that 44 of 460 samples (9.56%) were positive for h. pylori using the culture method. the polymerase chain reaction technique showed that 50 of 460 samples (10.86%) were positive for h. pylori. un-washed leek, traditional salad, un-washed basil and un...

abstract background brucellosis is endemic in iran, and is seen in all age groups, including children. its diagnosis in childhood needs a high index of suspicion. the diagnostic methods currently in use need evaluation for analytical performance. objective the present study tries to evaluate the hematological indices, serologic markers of inflammation and symptoms in patients suspected of bruce...

OBJECTIVE The aim of this study was to simultaneously monitoring cytomegalovirus and human herpesvirus 6 active infections using nested-polymerase chain reaction and, together with clinical findings, follow the clinical status of patients undergoing liver transplant. INTRODUCTION The human β-herpesviruses, including cytomegalovirus and human herpesvirus 6, are ubiquitous among human populatio...

Invasive aspergillosis (1 is a life-threatening condition in immunocompromised patients. An early diagnosis is of great importance because early treatment may resolve this potentially fatal infection. Recently, the polymerase chain reaction (PCR) has been used successfully in detecting specific DNA of several pathogen. In this study, nested PCR was used to detect DNA specific for A!.pergiflus s...

Nested Patch polymerase chain reaction (PCR) amplifies a large number (greater than 90) of targeted loci from genomic DNA simultaneously in the same reaction. These amplified loci can then be sequenced on a second-generation sequencing machine to detect single nucleotide polymorphisms (SNPs) and mutations. The reaction is highly specific: 90% of sequencing reads match targeted loci. Nested Patc...

Check out our Selection Guide! PCR RNA DNA LAMP KitsOne Tube For Superior Reproducibility. From fish tissues or fluids using a nested PCR primer set. The use of trade, firm, or corporation names in this protocol is for the information and.jor consideration in designing a nested PCR protocol compatible with quantitation is to assure that the maximum concentration of PCR products produced by the....

background: colorectal tumor is one of the main causes of death in our country. the aim of the present study was to determine the clinicopathological aspects of tumor and the presence of hepatic micrometastasis in patients with colorectal cancer (crc). materials and methods: forty two patients with crc were evaluated in the study surgical treatment was performed and liver biopsy was taken for t...

b ackground : one of the main mechanisms of resistance to carbapenems is potential of klebsiella pneumoniae to produce k . pneumoniae carbapenemase (kpc). kpc is an important type of carbapenemase, which can hydrolyze carbapenems and other beta-lactam antibiotics. modified hodge test (mht) and  use  of  boronic  acid  as  a  kpc  inhibitor  are  two  types  of  phenotypic methods, which are use...

conclusions: in neonates and infants with primary diagnosis of sepsis, hsv infection should be considered especially if the clinical condition does not improve after 48 hours of antibiotic therapy, and sepsis still exists with elevated liver enzymes. results: among 150 neonates and infants younger than 3 months old with sepsis, the pcr results for detecting the hsv dna, were positive in 6 sampl...

conclusions findings of this study showed that integron can have a limited frequency in s. aureus isolated from clinical sample in tehran. results among the 200 samples, 1% of the strains (two isolates) contained the class 1 integron gene. the results of this study showed that the highest frequency of the obtained samples belonged to males and the isolates occurred mostly in individuals aged 51...