To evaluate the Micral test, a semiquantitative dipstick test, in a general practice setting, 317 Type 2 diabetic patients completed a screening for microalbuminuria by means of the Micral test as well as immuno-nephelometry with the Disc 120 immuno-nephelometer (Hyland, Nivelles, Belgium). Data were collected in 10 general practices performing the Nijmegen Monitoring Project. At a regular chec...

Brillouin measurements of the elastic properties are presented for aligned multilamella samples of both pure dipalmitoyl phosphatidylcholine at various temperatures and water concentrations and for the same compound containing 33 mol% cholesterol at various temperatures and two water concentrations. For pure dipalmitoyl phosphatidylcholine the elastic moduli change significantly at the gel tran...

Using accelerated Arrhenius-type short-term and long-term temporal studies, we evaluated the storage life of a stabilized, liquid-frozen reference material (SLRM) for human apolipoprotein B (apo B) developed by the International Federation of Clinical Chemistry. As measured by our candidate reference RIA, the concentrations of immunoreactive apo B in the SLRM showed pronounced degradation with ...

The most cpmmon parameters in the specific protein field, namely the immunoglobulins G, A and M were investigated on the recently developed Immuno Video Nephelometer System (IVNS). This System consists of the nephelometer, a microprocessor controlled program and a monitor screen, where instructions, Standard curve and results are displayed. Scattered light of immuno-complexes is measured at equ...

We studied a serum component ("pseudolipase") that interferes with both turbidimetric and nephelometric lipase measurements. Pseudolipase activity in serum was found in the presence of rheumatoid factor and in other disorders of active immune processes. The incidence of this interferent in sera seems to be greater than reported previously. We describe a method for eliminating its effect without...

Measurement of immunoglobulins (Ig) and of complement component C3 in human serum by automated and manual nephelometric techniques is tedious, and the effective linear range is too narrow. We describe a laser nephelometer/reagent system for measuring serum proteins by the use of forward light scatter (which enhances the ratio of reaction/blank), electronic blank subtraction, laser light (632 nm...